Anti-TTF1 – Rabbit (WRAB-1231)使用说明
Anti-TTF1 – Rabbit (WRAB-1231)使用说明
产品信息
|
品牌 |
Seven Hills Bioreagents |
|
货号 |
WRAB-1231 |
|
规格 |
100 µL |
|
宿主 |
兔 |
|
反应种属 |
人、鼠 |
|
克隆类型 |
多克隆 |
|
偶联物 |
无 |
|
缓冲液 |
0.01% sodium azide |
|
抗原 |
重组鼠TTF-1融合6*his蛋白 |
|
保存条件 |
储存在-20℃，避免反复冻融 |
|
蛋白背景 |
甲状腺转录因子-1（TTF-1）是同源域转录因子家族的成员，并且在调节甲状腺，肺和脑内表达的基因中起作用。 |
产品使用
|
实验应用 |
建议稀释倍数 |
|
Western Blot |
1: 5000 |
|
Immunohistochemistry |
1:1000 – 1:2000 |
说明：最佳稀释倍数与浓度应由研究人员确认
应用实例
图片为成年小鼠肺组织，用6%正常山羊血清预包埋，并用抗大鼠TTF-1兔血清（1:2000稀释）染色。通过DAB（黑色）观察TTF-1，并用核快红（粉红色）复染。放大倍数10倍。
参考文献
1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.
3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.
4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
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