| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0013 | pcDNA4/HisMax B | Invitrogen | 哺乳动物表达载体 |
Mammalian Selection：Zeocin
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′
Tag：6X His，Xpress
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0017 | pcDNA3.1（-）/myc-His A | Invitrogen | 哺乳动物表达载体 |
| VPI0018 | pcDNA3.1（-）/myc-His B | Invitrogen | 哺乳动物表达载体 |
| VPI0019 | pcDNA3.1（-）/myc-His C | Invitrogen | 哺乳动物表达载体 |
Mammalian Selection：G418，neo
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His）
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0010 | pcDNA3.1/GS | Invitrogen | 哺乳动物表达载体 |
Mammalian Selection：Zeocin
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0009 | pcDNA3.1（zeo）+ | Invitrogen | 哺乳动物表达载体 |
Mammalian Selection：Zeocin
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VPI0008 | pcDNA3.1（hyg）+ | Invitrogen | 哺乳动物表达载体 |
Promotor: CMV
5′ Sequencing 1 Primer: T7 Fwd
5′ Sequencing 1 Primer Sequence: 5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker: Hygromycin
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VGI0404 | pcDNA6.2-GWEmGFP-miR negative | Invitrogen | Gateway系统 |
Comments for:
CMV promoter:
attB1 site:
EmGFP:
EmGFP forward sequencing primer site:
5’ miR flanking region:
miR-neg control:
3’ miR flanking region:
attB2 site （c）:
miRNA reverse sequencing primer site （c）:
TK polyadenylation signal:
f1 origin:
SV40 early promoter and origin:
EM7 promoter:
Blasticidin resistance gene:
SV40 polyadenylation signal:
pUC origin （c）:
Spectinomycin resistance gene （c）:
Spect ni omycin
5’ miR flanking
region attB1 EmGFP attB2
pcDNATM 6.2-GW/EmGFP-miR-neg
control plasmid
5759 nucleotides
1 – 588
680 – 704
713 – 1432
1409 – 1428
1492 – 1518
1519 – 1578
1579 – 1623
1652 – 1676
1667 – 1686
1705 – 1976
2088 – 2516
2543 – 2851
2906 – 2972
2973 – 3371
3529 – 3659
3797 – 4470
4540 – 5550
Invitrogen pcDNA4 TO Myc-His LacZ 四环素诱导系统说明书
pcDNA™6⁄TR
载体描述:
The pcDNA™6⁄TR vector is part of the T-REx™ System, which yields higher levels of induced expression than any other regulated mammalian expression system. It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon to effectively repress and derepress transcription from one of the strongest mammalian promoter sequences known.
The regulatory vector, pcDNA™6⁄TR, is provided for high-level expression of the tetracycline repressor （TR） protein. This vector expresses the Blasticidin resistance gene for rapid selection of mammalian cell lines that stably express the TR protein.
Invitrogen四环素诱导系统
Vectors：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，
产品参数:
Mammalian Selection：Zeocin
Sequencing Primer：T7 Fwd
Sequencing Primer Sequence：5’d[TAATACGACTCACTATAGGG]3′
Tag：6X His，Xpress