标签归档：luc

PG13-luc (wt p53 binding sites)（萤火虫荧光素酶报告基因质粒）说明书

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PG13-luc (wt p53 binding sites)（萤火虫荧光素酶报告基因质粒）说明书

PG13-luc (wt p53 binding sites)（萤火虫荧光素酶报告基因质粒）说明书

产品详情  

货号

规格

价格

78BDB10012-2ug

2ug

￥2000.00

质粒图谱

 

载体基本信息

质粒名称:

 

PG13-luc (wt p53 binding sites)

载体骨架基本信息

载体名称:

pBluescript II SK + luc

载体抗性:

 

氨苄青霉素

空载体大小:

5800 bp

载体类型:

哺乳动物表达，荧光素酶

生长条件:

37 ℃

插入基因信息

插入基因名称:

p53-binding site

标签/融合蛋白:

luciferase（C端在主干上）

克隆方法:

限制性内切酶

5' 克隆位点:

HindIII（如果被摧毁则未知）

3' 克隆位点:

 

EcoRI（如果被摧毁则未知）

5' 测序引物

T7

3' 测序引物

LucNrev

文献引用方法

材料和方法部分:

PG13-luc (wt p53 binding sites) was a gift from Bert Vogelstein (Addgene plasmid # 16442 ; http://n2t.net/addgene:16442 ; RRID:Addgene_16442)

参考文献部分

WAF1, a potential mediator of p53 tumor suppression. el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B. Cell. 1993 Nov 19. 75(4):817-25. 10.1016/0092-8674(93)90500-P PubMed 8242752

pSRE-Luc载体说明书

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 pSRE-Luc

型号 载体名称 出品公司 载体用途
VXS0449 pSRE-Luc Stratagene 信号通路报告载体

Instruction Manual

Catalog #219073 （AP-1 cis-Reporting System） #219075 （CRE cis-Reporting System） 

#219077 （NF-κB cis-Reporting System） #219079 （SRE cis-Reporting System） 

#219081 （SRF cis-Reporting System） #219083 （p53 cis-Reporting System） 

#219092 （ISRE cis-Reporting System） #219093 （GAS cis-Reporting System） 

#219094 （NFAT cis-Reporting System） #219095 （TARE cis-Reporting System） 

#240111 （C/EBP cis-Reporting System） #240113 （DR1 cis-Reporting System） 

#240115 （DR3 cis-Reporting System） #240119 （DR5 cis-Reporting System） 

#240129 （Egr-1 cis-Reporting System） #240131 （LILRE cis-Reporting System） 

#240133 （GRE cis-Reporting System） #240135 （DR4 cis-Reporting System） 

#240049 （pAP-1-hrGFP Plasmid） #240050 （pCRE-hrGFP Plasmid） 

#240051 （pNF-κB-hrGFP Plasmid） #240053 （pNFAT-hrGFP Plasmid） 

#219087 （pLuc-MCS Plasmid） 

质粒图谱: 

pNF-κB-Luc载体说明书

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 pNF-κB-Luc

型号 载体名称 出品公司 载体用途
VXS0448 pNF-κB-Luc Stratagene 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: 

p53-Luc载体说明书

发表于由

 p53-Luc

型号 载体名称 出品公司 载体用途
VXS0446 p53-Luc Stratagene 信号通路报告载体

Instruction Manual

Catalog #219073 （AP-1 cis-Reporting System） #219075 （CRE cis-Reporting System） 

#219077 （NF-κB cis-Reporting System） #219079 （SRE cis-Reporting System） 

#219081 （SRF cis-Reporting System） #219083 （p53 cis-Reporting System） 

#219092 （ISRE cis-Reporting System） #219093 （GAS cis-Reporting System） 

#219094 （NFAT cis-Reporting System） #219095 （TARE cis-Reporting System） 

#240111 （C/EBP cis-Reporting System） #240113 （DR1 cis-Reporting System） 

#240115 （DR3 cis-Reporting System） #240119 （DR5 cis-Reporting System） 

#240129 （Egr-1 cis-Reporting System） #240131 （LILRE cis-Reporting System） 

#240133 （GRE cis-Reporting System） #240135 （DR4 cis-Reporting System） 

#240049 （pAP-1-hrGFP Plasmid） #240050 （pCRE-hrGFP Plasmid） 

#240051 （pNF-κB-hrGFP Plasmid） #240053 （pNFAT-hrGFP Plasmid） 

#219087 （pLuc-MCS Plasmid）

质粒图谱: 

pGRE-Luc载体说明书

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 pGRE-Luc

型号 载体名称 出品公司 载体用途
VXC0430 pGRE-Luc Clontech 信号通路报告载体

 

Instruction Manual

Catalog #219073 （AP-1 cis-Reporting System） #219075 （CRE cis-Reporting System） 

#219077 （NF-κB cis-Reporting System） #219079 （SRE cis-Reporting System） 

#219081 （SRF cis-Reporting System） #219083 （p53 cis-Reporting System） 

#219092 （ISRE cis-Reporting System） #219093 （GAS cis-Reporting System） 

#219094 （NFAT cis-Reporting System） #219095 （TARE cis-Reporting System） 

#240111 （C/EBP cis-Reporting System） #240113 （DR1 cis-Reporting System） 

#240115 （DR3 cis-Reporting System） #240119 （DR5 cis-Reporting System） 

#240129 （Egr-1 cis-Reporting System） #240131 （LILRE cis-Reporting System） 

#240133 （GRE cis-Reporting System） #240135 （DR4 cis-Reporting System） 

#240049 （pAP-1-hrGFP Plasmid） #240050 （pCRE-hrGFP Plasmid） 

#240051 （pNF-κB-hrGFP Plasmid） #240053 （pNFAT-hrGFP Plasmid） 

#219087 （pLuc-MCS Plasmid）

 

质粒图谱: 

 

 

pCRE-Luc载体说明书

发表于由

pCRE-Luc

型号 载体名称 出品公司 载体用途
VXC0428 pCRE-Luc Clontech 信号通路报告载体

 

The Agilent PathDetect in Vivo Signal Transduction Pathway cis-Reporting 

Systems are designed for simple, rapid, and convenient assessment of the in 

vivo activation of signal transduction pathways. We have created a series of 

inducible reporter plasmids that contain the luciferase reporter gene driven 

by a basic promoter element （TATA box） plus a defined inducible cisenhancer element （see Figure 2 in Appendix）. These plasmids are 

particularly suited for the in vivo readouts of signal transduction pathways 

since these enhancers are convergent points of many signal transduction 

pathways. These systems are useful in studying the in vivo effects of a new 

gene, growth factor, or drug candidate on the signaling pathway. Some of 

the reporters may also be used for cloning novel signal transduction genes, 

and may be useful for identifying drug candidates in a high-throughput 

format. 

质粒图谱: 

pAP1（PMA）-TA-Luc载体说明书

发表于由

 pAP1（PMA）-TA-Luc

型号 载体名称 出品公司 载体用途
VXC0437 pAP1（PMA）-TA-Luc Clontech 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: 

pNFAT-TA-Luc载体说明书

发表于由

 pNFAT-TA-Luc

型号 载体名称 出品公司 载体用途
VXC0436 pNFAT-TA-Luc Clontech 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: 

pTA-Luc载体说明书

发表于由

 pTA-Luc载体

型号 载体名称 出品公司 载体用途
VXC0434 pTA-Luc Clontech 信号通路报告载体
产品参数: 

Plasmid Type: Mammalian Expression
Promotor: TATA box
Stable: Transient
Constitutive: Minimal or No Promoter
Viral/Non-Viral: Nonviral

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Luciferase reporter. For studying the transcriptional activity of enhancers.

质粒图谱: 

pISRE-TA-Luc载体说明书

发表于由

 pISRE-TA-Luc

型号 载体名称 出品公司 载体用途
VXC0433 pISRE-TA-Luc Clontech 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: 
 

pSTAT3-TA-Luc载体说明书

发表于由

 pSTAT3-TA-Luc

型号 载体名称 出品公司 载体用途
VXC0432 pSTAT3-TA-Luc Clontech 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: 

pGAS-TA-Luc 载体说明书

发表于由

 pGAS-TA-Luc 载体

型号 载体名称 出品公司 载体用途
VXC0429 pGAS-TA-Luc Clontech 信号通路报告载体

The Pathway Profiling Systems allow you to quickly profile the effects of 

a given stimulus, drug candidate, or gene of interest on key signal transduction pathways in vivo. The Pathway Profiling Systems cover a variety 

of signal transduction pathways in eukaryotic cells. Pathway Profiling Systems are available for broad-spectrum or targeted profiling of key signaling 

pathways:

• SEAP System—broad coverage

• Luciferase System 4—cell cycle

质粒图谱: