Nanobind UL Library Prep Kit即将上市，敬请期待！
circulomics即将推出的Nanobind UL库准备套件，用于超长纳米孔测序，我们与牛津纳米孔合作开发。
Nanobind UL库准备套件（NB-900-601-01）零售价为180美元。它与ONT超长DNA测序试剂盒一起用于反应纯化，以运行Nanobind增强超长测序协议。
该方法可以获得50-100kb的读取长度N50，MinION上的吞吐量为10-20+Gb，PromethION上的吞吐量为40-100+Gb，长读取可达4mb。
每个Nanobind UL库准备套件还将配备一个UHMW细胞或血液辅助包（客户根据样本类型选择所需）。Aux包与Nanobind CBB Big DNA试剂盒一起使用，以运行我们专门为这种测序方法开发的新UHMW DNA提取协议。
circulomics NB-900-601-01
附件为将circulomics在ONT NCM上展示的海报。产品图片如上。
Bacteria Resistance：KanamycinMammalian Selection：-
载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。
pGBKT7-53 encodes a fusion of the murine p53 protein （a.a. 72–390） and the GAL4 DNA-BD （a.a. 1–147）. The murine p53 cDNA （GenBank Accession #K01700） was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. （1993）; plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.
Bacteria Resistance：KanamycinMammalian Selection：-
载体特点：Matchmaker Two-Hybrid System 3的负对照。
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein （a.a. 66–230） and the GAL4 DNA-BD （a.a. 1–147）. The lamin C cDNA insert （GenBank Accession #M13451） was derived from the plasmid referenced in Bar et al. （1993a）. Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.