2-Aminoethoxypropargyl ddATP
 |
货号 |
17084 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
1 umoles |
价格 |
11988 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
659.24 |
溶剂 |
DMF |
| 产品详细介绍 |
简要概述
产品基本信息
货号：17084
产品名称：2-Aminoethoxypropargyl ddATP
规格：1 umoles
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：659.24
外观：液体
溶剂：DMF
激发波长(nm)：N/A
发射波长(nm)：N/A
产品介绍
Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的2-Aminoethoxypropargyl ddATP。
参考文献
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Journal: Nucleic Acids Res (2000): 1059-66
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
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Journal: Proc Natl Acad Sci U S A (1999): 9491-6
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
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Journal: Biochemistry (1998): 10529-39
Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
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Journal: AIDS Res Hum Retroviruses (1998): 869-76
说明书
2-Aminoethoxypropargyl ddATP.pdf
