ClodronateLiposomes2021年价格价格表

ClodronateLiposomes2021年价格价格表

氯膦酸二钠脂质体(ClodronateLiposomes & PBS Lipomes)是用来在实验动物体内(invivo)或者体外(invritro)清余(Deplete)单核巨噬细胞的即用型液体试剂。PEs脂质体是氯膦酸二钠脂质体的严格对照。
脂质体(Liposones)是一种人工膜,由磷脂和胆固醇构成。一般直径在25nm-lum之间。细胞体外(invitro)吞嗌实验显示,随着脂质体(Liposome)的直径从100nn到1um,细胞吞噬的脂质体(Liposomes)随之增多,直至高达2un;动物体内(invivo)实验显示,随着直径增大,体内香噬的脂质体(Liposomes)更多。脂质体(Liposomes)和巨噬细胞‘(lMacxrophages)间有最小的间隙,辅以改变脂质体(Liposones)表面的电荷,尤其是负电荷,能更好促进巨噬细胞对脂质体的吞噬。采用多室的脂质体结构,让包裹更多的药物,从而到达效应的阈浓度。
氯膦酸二钠(Clodronate),化学名为:二氯亚甲基二膦酸二钠,在细胞内代谢成不可水解的ATP类似物,通过抑制线粒体中的ADP/ATP转运机制诱导细胞凋亡。
L.TPOSOMA优化脂质体的组分,充分利用脂质体和巨噬细胞的属性,改进脂质体包裹氯膦酸二钠的工艺,严格控制产品的封装效率,粒径分布,无菌操作等关键参数,确保产品的批内稳定性和批间重复性。

ClodronateLiposomes2021年价格价格表

 

货号 品名 规格 价格 品牌
C-005 CLODRONATE LIPOSOMES 5 ml 4500 ClodronateLiposomes2021
C-010 CLODRONATE LIPOSOMES 10 ml 7500 ClodronateLiposomes2021
C-015 CLODRONATE LIPOSOMES 15 ml 10500 ClodronateLiposomes2021
C-020 CLODRONATE LIPOSOMES 20 ml 13500 ClodronateLiposomes2021
C-030 CLODRONATE LIPOSOMES 30 ml 18750 ClodronateLiposomes2021
C-040 CLODRONATE LIPOSOMES 40 ml 24000 ClodronateLiposomes2021
C-050 CLODRONATE LIPOSOMES 50 ml 28500 ClodronateLiposomes2021
C-100 CLODRONATE LIPOSOMES 100 ml 52500 ClodronateLiposomes2021
CP-005-005 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 5 ml + 5 ml 6000 ClodronateLiposomes2021
CP-010-010 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 10 ml + 10 ml 10000 ClodronateLiposomes2021
CP-015-015 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 15 ml + 15 ml 14000 ClodronateLiposomes2021
CP-020-020 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 20 ml + 20 ml 18000 ClodronateLiposomes2021
CP-030-030 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 30 ml + 30 ml 25000 ClodronateLiposomes2021
CP-040-040 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 40 ml + 40 ml 32000 ClodronateLiposomes2021
CP-050-050 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 50 ml + 50 ml 38000 ClodronateLiposomes2021
CP-100-100 CLODRONATE LIPOSOMES AND PBS LIPOSOMES 100 ml + 100 ml 70000 ClodronateLiposomes2021
C-SUV-005 CLODRONATE NANO LIPOSOMES 5 ml 5250 ClodronateLiposomes2021
C-SUV-010 CLODRONATE NANO LIPOSOMES 10 ml 9000 ClodronateLiposomes2021
C-SUV-015 CLODRONATE NANO LIPOSOMES 15 ml 12750 ClodronateLiposomes2021
C-SUV-020 CLODRONATE NANO LIPOSOMES 20 ml 16500 ClodronateLiposomes2021
C-SUV-030 CLODRONATE NANO LIPOSOMES 30 ml 22500 ClodronateLiposomes2021
C-SUV-040 CLODRONATE NANO LIPOSOMES 40 ml 28500 ClodronateLiposomes2021
C-SUV-050 CLODRONATE NANO LIPOSOMES 50 ml 33750 ClodronateLiposomes2021
C-SUV-100 CLODRONATE NANO LIPOSOMES 100 ml 63000 ClodronateLiposomes2021
CP-SUV-005-005 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 5 ml + 5 ml 7000 ClodronateLiposomes2021
CP-SUV-010-010 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 10 ml + 10 ml 12000 ClodronateLiposomes2021
CP-SUV-015-015 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 15 ml + 15 ml 17000 ClodronateLiposomes2021
CP-SUV-020-020 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 20 ml + 20 ml 22000 ClodronateLiposomes2021
CP-SUV-030-030 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 30 ml + 30 ml 30000 ClodronateLiposomes2021
CP-SUV-040-040 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 40 ml + 40 ml 38000 ClodronateLiposomes2021
CP-SUV-050-050 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 50 ml + 50 ml 45000 ClodronateLiposomes2021
CP-SUV-100-100 CLODRONATE NANO LIPOSOMES AND PBS LIPOSOMES 100 ml + 100 ml 84000 ClodronateLiposomes2021
C-SUV–P-005 CLODRONATE NANO PEG LOADED LIPOSOMES 5 ml 6000 ClodronateLiposomes2021
C-SUV-P-010 CLODRONATE NANO PEG LOADED LIPOSOMES 10 ml 11250 ClodronateLiposomes2021
C-SUV-P-015 CLODRONATE NANO PEG LOADED LIPOSOMES 15 ml 16500 ClodronateLiposomes2021
C-SUV-P-020 CLODRONATE NANO PEG LOADED LIPOSOMES 20 ml 21750 ClodronateLiposomes2021
C-SUV-9-030 CLODRONATE NANO PEG LOADED LIPOSOMES 30 ml 29250 ClodronateLiposomes2021
C-SUV-P-040 CLODRONATE NANO PEG LOADED LIPOSOMES 40 ml 36750 ClodronateLiposomes2021
C-SUV-P-050 CLODRONATE NANO PEG LOADED LIPOSOMES 50 ml 45000 ClodronateLiposomes2021
C-SUV-P-100 CLODRONATE NANO PEG LOADED LIPOSOMES 100 ml 79500 ClodronateLiposomes2021
CP-SUV-P-005-005 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 5 ml + 5 ml 8000 ClodronateLiposomes2021
CP-SUV-P-010-010 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 10 ml + 10 ml 15000 ClodronateLiposomes2021
CP-SUV-P-020-020 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 15 ml + 15 ml 22000 ClodronateLiposomes2021
CP-SUV-P-020-020 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 20 ml + 20 ml 29000 ClodronateLiposomes2021
CP-SUV-P-030-030 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 30 ml + 30 ml 39000 ClodronateLiposomes2021
CP-SUV-P-040-040 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 40 ml + 40 ml 49000 ClodronateLiposomes2021
CP-SUV-P-050-050 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 50 ml + 50 ml 60000 ClodronateLiposomes2021
CP-SUV-P-100-100 CLODRONATE NANO PEG LOADED AND PBS LIPOSOMES 100 ml + 100 ml 106000 ClodronateLiposomes2021
P-005 CONTROL (PBS) LIPOSOMES 5 ml 1500 ClodronateLiposomes2021
P-010 CONTROL (PBS) LIPOSOMES 10 ml 2500 ClodronateLiposomes2021
P-015 CONTROL (PBS) LIPOSOMES 15 ml 3500 ClodronateLiposomes2021
P-020 CONTROL (PBS) LIPOSOMES 20 ml 4500 ClodronateLiposomes2021
P-030 CONTROL (PBS) LIPOSOMES 30 ml 6250 ClodronateLiposomes2021
P-040 CONTROL (PBS) LIPOSOMES 40 ml 8000 ClodronateLiposomes2021
P-050 CONTROL (PBS) LIPOSOMES 50 ml 9500 ClodronateLiposomes2021
P-100 CONTROL (PBS) LIPOSOMES 100 ml 17500 ClodronateLiposomes2021
P-SUV-005 CONTROL NANO (PBS) LIPOSOMES 5 ml 1750 ClodronateLiposomes2021
P-SUV-010 CONTROL NANO (PBS) LIPOSOMES 10 ml 3000 ClodronateLiposomes2021
P-SUV-015 CONTROL NANO (PBS) LIPOSOMES 15 ml 4250 ClodronateLiposomes2021
P-SUV-020 CONTROL NANO (PBS) LIPOSOMES 20 ml 5500 ClodronateLiposomes2021
P-SUV-030 CONTROL NANO (PBS) LIPOSOMES 30 ml 7500 ClodronateLiposomes2021
P-SUV-040 CONTROL NANO (PBS) LIPOSOMES 40 ml 9500 ClodronateLiposomes2021
P-SUV-050 CONTROL NANO (PBS) LIPOSOMES 50 ml 11250 ClodronateLiposomes2021
P-SUV-100 CONTROL NANO (PBS) LIPOSOMES 100 ml 21000 ClodronateLiposomes2021
P-SUV-P-005 CONTROL NANO (PBS) LIPOSOMES 5 ml 2000 ClodronateLiposomes2021
P-SUV-P-010 CONTROL NANO (PBS) LIPOSOMES 10 ml 3750 ClodronateLiposomes2021
P-SUV-P-015 CONTROL NANO (PBS) LIPOSOMES 15 ml 5500 ClodronateLiposomes2021
P-SUV-P-020 CONTROL NANO (PBS) LIPOSOMES 20 ml 7250 ClodronateLiposomes2021
P-SUV-P-030 CONTROL NANO (PBS) LIPOSOMES 30 ml 9750 ClodronateLiposomes2021
P-SUV-P-040 CONTROL NANO (PBS) LIPOSOMES 40 ml 12250 ClodronateLiposomes2021
P-SUV-P-050 CONTROL NANO (PBS) LIPOSOMES 50 ml 15000 ClodronateLiposomes2021
P-SUV-P-100 CONTROL NANO (PBS) LIPOSOMES 100 ml 26500 ClodronateLiposomes2021

clodronateliposomes脂质体2020年价格表

clodronateliposomes脂质体2020年价格表

 

clodronateliposomes脂质体2020年价格表

 

货号 品名   规格   价格  
C-005-005 Clodronate Liposomes 5 ml 4100
C-010-010 Clodronate Liposomes 10 ml 6400
C-015-015 Clodronate Liposomes 15 ml 9000
C-020-020 Clodronate Liposomes 20 ml 11500
C-025-025 Clodronate Liposomes 25 ml 13800
C-030-030 Clodronate Liposomes 30 ml 16100
C-040-040 Clodronate Liposomes 40 ml 20700
C-050-050 Clodronate Liposomes 50 ml 25300
C-060-060 Clodronate Liposomes 60 ml 28700
C-070-070 Clodronate Liposomes 70 ml 32200
C-080-080 Clodronate Liposomes 80 ml 35600
C-090-090 Clodronate Liposomes 90 ml 38000
C-100-100 Clodronate Liposomes 100 ml 40300
C-150-150 Clodronate Liposomes 150 ml 60400
C-200-200 Clodronate Liposomes 200 ml 80500
C-250-250 Clodronate Liposomes 250 ml 100600
C-300-300 Clodronate Liposomes 300 ml 120700
C-350-350 Clodronate Liposomes 350 ml 140900
C-400-400 Clodronate Liposomes 400 ml 161000
C-450-450 Clodronate Liposomes 450 ml 181100
C-500-500 Clodronate Liposomes 500 ml 201200
P-005-005 Control Liposomes (PBS) 5 ml 1700
P-010-010 Control Liposomes (PBS) 10 ml 3000
P-015-015 Control Liposomes (PBS) 15 ml 4100
P-020-020 Control Liposomes (PBS) 20 ml 5100
P-025-025 Control Liposomes (PBS) 25 ml 6000
P-030-030 Control Liposomes (PBS) 30 ml 6900
P-040-040 Control Liposomes (PBS) 40 ml 8300
P-050-050 Control Liposomes (PBS) 50 ml 9700
P-060-060 Control Liposomes (PBS) 60 ml 11000
P-070-070 Control Liposomes (PBS) 70 ml 12200
P-080-080 Control Liposomes (PBS) 80 ml 13300
P-090-090 Control Liposomes (PBS) 90 ml 14500
P-100-100 Control Liposomes (PBS) 100 ml 15600
P-150-150 Control Liposomes (PBS) 150 ml 23500
P-200-200 Control Liposomes (PBS) 200 ml 31300
P-250-250 Control Liposomes (PBS) 250 ml 39100
P-300-300 Control Liposomes (PBS) 300 ml 46900
P-350-350 Control Liposomes (PBS) 350 ml 54700
P-400-400 Control Liposomes (PBS) 400 ml 62600
P-450-450 Control Liposomes (PBS) 450 ml 70400
P-500-500 Control Liposomes (PBS) 500 ml 78200
I-005-005 Fluorescent Dil Liposomes 5 ml 2400
I-010-010 Fluorescent Dil Liposomes 10 ml 4400
I-015-015 Fluorescent Dil Liposomes 15 ml 6200
I-020-020 Fluorescent Dil Liposomes 20 ml 7600
I-025-025 Fluorescent Dil Liposomes 25 ml 9000
I-030-030 Fluorescent Dil Liposomes 30 ml 10400
I-040-040 Fluorescent Dil Liposomes 40 ml 12400
I-050-050 Fluorescent Dil Liposomes 50 ml 14500
I-060-060 Fluorescent Dil Liposomes 60 ml 16600
I-070-070 Fluorescent Dil Liposomes 70 ml 18400
I-080-080 Fluorescent Dil Liposomes 80 ml 20200
I-090-090 Fluorescent Dil Liposomes 90 ml 22000
I-100-100 Fluorescent Dil Liposomes 100 ml 23900
I-150-150 Fluorescent Dil Liposomes 150 ml 35900
I-200-200 Fluorescent Dil Liposomes 200 ml 47800
I-250-250 Fluorescent Dil Liposomes 250 ml 59800
I-300-300 Fluorescent Dil Liposomes 300 ml 71700
I-350-350 Fluorescent Dil Liposomes 350 ml 83700
I-400-400 Fluorescent Dil Liposomes 400 ml 95700
I-450-450 Fluorescent Dil Liposomes 450 ml 107600
I-500-500 Fluorescent Dil Liposomes 500 ml 119600
CP-005-005 Clodronate Liposomes & Control Liposomes (PBS) 5 ml 5800
CP-010-010 Clodronate Liposomes & Control Liposomes (PBS) 10 ml 9400
CP-015-015 Clodronate Liposomes & Control Liposomes (PBS) 15 ml 13100
CP-020-020 Clodronate Liposomes & Control Liposomes (PBS) 20 ml 16600
CP-025-025 Clodronate Liposomes & Control Liposomes (PBS) 25 ml 19800
CP-030-030 Clodronate Liposomes & Control Liposomes (PBS) 30 ml 23000
CP-040-040 Clodronate Liposomes & Control Liposomes (PBS) 40 ml 29000
CP-050-050 Clodronate Liposomes & Control Liposomes (PBS) 50 ml 35000
CP-060-060 Clodronate Liposomes & Control Liposomes (PBS) 60 ml 39700
CP-070-070 Clodronate Liposomes & Control Liposomes (PBS) 70 ml 44400
CP-080-080 Clodronate Liposomes & Control Liposomes (PBS) 80 ml 48900
CP-090-090 Clodronate Liposomes & Control Liposomes (PBS) 90 ml 52500
CP-100-100 Clodronate Liposomes & Control Liposomes (PBS) 100 ml 55900
CP-150-150 Clodronate Liposomes & Control Liposomes (PBS) 150 ml 83900
CP-200-200 Clodronate Liposomes & Control Liposomes (PBS) 200 ml 111800
CP-250-250 Clodronate Liposomes & Control Liposomes (PBS) 250 ml 139700
CP-300-300 Clodronate Liposomes & Control Liposomes (PBS) 300 ml 167600
CP-350-350 Clodronate Liposomes & Control Liposomes (PBS) 350 ml 195600
CP-400-400 Clodronate Liposomes & Control Liposomes (PBS) 400 ml 223600
CP-450-450 Clodronate Liposomes & Control Liposomes (PBS) 450 ml 251500
CP-500-500 Clodronate Liposomes & Control Liposomes (PBS) 500 ml 279400
CI-005-005 Clodronate Liposomes &Fluorescent Dil Liposomes 5 ml 6500
CI-010-010 Clodronate Liposomes &Fluorescent Dil Liposomes 10 ml 10800
CI-015-015 Clodronate Liposomes &Fluorescent Dil Liposomes 15 ml 15200
CI-020-020 Clodronate Liposomes &Fluorescent Dil Liposomes 20 ml 19100
CI-025-025 Clodronate Liposomes &Fluorescent Dil Liposomes 25 ml 22800
CI-030-030 Clodronate Liposomes &Fluorescent Dil Liposomes 30 ml 26500
CI-040-040 Clodronate Liposomes &Fluorescent Dil Liposomes 40 ml 33100
CI-050-050 Clodronate Liposomes &Fluorescent Dil Liposomes 50 ml 39800
CI-060-060 Clodronate Liposomes &Fluorescent Dil Liposomes 60 ml 45300
CI-070-070 Clodronate Liposomes &Fluorescent Dil Liposomes 70 ml 50600
CI-080-080 Clodronate Liposomes &Fluorescent Dil Liposomes 80 ml 55800
CI-090-090 Clodronate Liposomes &Fluorescent Dil Liposomes 90 ml 60000
CI-100-100 Clodronate Liposomes &Fluorescent Dil Liposomes 100 ml 64200
CI-150-150 Clodronate Liposomes &Fluorescent Dil Liposomes 150 ml 96300
CI-200-200 Clodronate Liposomes &Fluorescent Dil Liposomes 200 ml 128300
CI-250-250 Clodronate Liposomes &Fluorescent Dil Liposomes 250 ml 160400
CI-300-300 Clodronate Liposomes &Fluorescent Dil Liposomes 300 ml 192400
CI-350-350 Clodronate Liposomes &Fluorescent Dil Liposomes 350 ml 224600
CI-400-400 Clodronate Liposomes &Fluorescent Dil Liposomes 400 ml 256700
CI-450-450 Clodronate Liposomes &Fluorescent Dil Liposomes 450 ml 288700
CI-500-500 Clodronate Liposomes &Fluorescent Dil Liposomes 500 ml 320800

 

 

clodronateliposomes脂质体使用说明书

clodronateliposomes脂质体使用说明书

clodronateliposomes脂质体使用说明书

 

产品描述

磷脂双层由磷脂酰胆碱和胆固醇组成。将所有脂质体制剂悬浮在无菌磷酸盐缓冲盐水(PBS)中:

– 10 mM的Na2HPO4;
– 10毫米NaH2PO4;
– 140毫米氯化钠。

我们所有的脂质体均带负电荷,因此不会一起阻塞。悬浮液中的脂质体没有大小,这意味着它也包含越来越大的脂质体(大3微米)。平均而言,脂质体的大小为1.7微米。

氯膦酸盐脂质体:人工制备的脂质囊泡的悬浮液,包裹了氯膦酸盐。悬浮液中氯膦酸盐的浓度约为。5毫克/毫升 氯膦酸盐以CH2Na2Cl2O6P2·5 H2O的形式封装在脂质体囊泡中。

PBS脂质体:人工制备的脂质囊泡的悬浮液,其中包裹了PBS水溶液。这些不含氯膦酸盐,可用于对照实验。

荧光DiI脂质体:人工制备的脂质囊泡的悬浮液,其中包封了PBS水溶液,并用荧光染料DiI标记。它们不含氯膦酸盐,可用于研究通过特定给药途径注射的脂质体是否能够到达要研究的巨噬细胞。

 

储存和使用说明

到达脂质体后,应将其储存在4 – 8ºC(或39 – 47ºF)之间。脂质体悬浮液不得冷冻,也不要暴露于高温下。这可能会对磷脂双层造成干扰,可能导致氯膦酸盐从脂质体中泄漏出来。

给药前,首先使脂质体达到室温,然后轻轻摇动或搅拌悬浮液。一段时间后,脂质体易于沉淀,导致小瓶中的分布不均匀。当注射花费太多时间时,脂质体甚至可能在注射器中沉淀。如果使用同一注射器注射多只动物,可能会导致剂量不同。

不建议稀释悬浮液,但如有必要,请使用PBS或盐水。

我们建议客户在发货后16周内使用脂质体制剂。强烈建议不要在到期日期之后使用。在此期间之后,污染的风险会增加,并且可能会发生轻微的功能丧失。

作用机理

巨噬细胞在免疫和非免疫防御机制中起重要作用。它们是抵御渗透到脊椎动物体内的细菌,病毒和其他形式的微生物污染的首道防线。巨噬细胞是大型细胞,几乎存在于所有身体组织中,它们可以具有不同的形式和名称(例如库普弗细胞,肺泡巨噬细胞,小胶质细胞,破骨细胞,红髓巨噬细胞)。巨噬细胞“清除”,它们摄入并消化可能是潜在病原体的所有异物,微生物,癌细胞和细胞碎片。该过程称为吞噬作用。巨噬细胞主要通过可溶性分子如细胞因子和趋化因子的介导来进一步调节许多非吞噬细胞的功能。他们参与先天免疫,

脂质体是人工制备的球体,由同心磷脂双层组成。当磷脂分散在水中时,亲水性头部将构成脂质体的两个外部部分,而疏水性脂肪酸基团将构成内部部分(见图1)。水室分隔双层,亲水分子可以溶解在其中,形成脂质体包封的分子。氯膦酸盐(二氯亚甲基双膦酸酯或Cl2MBP)是一种亲水性分子,可以封装在磷脂双层中。游离氯膦酸盐不易穿过细胞膜,并通过肾脏系统从循环中迅速清除(即数分钟内)。但是,氯膦酸盐脂质体包埋在脂质体中时,会被巨噬细胞吞噬,无法逃脱(见图2)。磷脂双层被溶酶体磷脂酶消化,而氯膦酸盐未被消化并且保留在巨噬细胞中。巨噬细胞摄入的磷脂双层和脂质体越多,在巨噬细胞内积累的氯膦酸盐越多。超过一定的细胞内浓度,氯膦酸盐将通过启动程序性细胞死亡(即凋亡)来消除巨噬细胞。

图1.氯膦酸盐脂质体的示意图。囊泡由同心的磷脂双层构成,被水性隔室隔开。双层由亲水和疏水基团组成。将氯膦酸盐(在此以黑色方块表示)溶解在水溶液中并封装在脂质体内。

 

图2.巨噬细胞对氯膦酸盐脂质体的摄取和消化示意图。巨噬细胞通过胞吞作用摄入氯膦酸盐脂质体,然后与含有磷脂酶的溶酶体(L)融合(箭头)。磷脂酶破坏的磷脂双层越多,在巨噬细胞内释放的氯膦酸盐(黑色方块)越多。巨噬细胞终通过凋亡被杀死。(N =巨噬细胞核)。

 
因此,氯膦酸盐脂质体可用于通过消耗巨噬细胞来研究巨噬细胞功能。例如,它们可以应用于各种自身免疫性疾病,移植,神经系统疾病和基因治疗的模型。氯膦酸盐脂质体只有在可以达到的情况下才能耗尽巨噬细胞。一些组织可以形成脂质体的屏障。通过选择氯膦酸盐脂质体的正确给药途径,可以耗尽特定的器官或组织的巨噬细胞。有关管理协议的更多信息,请单击此处。

PBS脂质体主要用于对照实验。但是,这些也可以在某些时间段内通过饱和来阻止吞噬作用。因此,PBS脂质体不能代表正常健康的,未阻断的,未抑制的和未活化的巨噬细胞的对照实验。当比较氯膦酸盐脂质体和PBS脂质体的作用时,其作用因此可能小于预期。

有关更多信息,请参见:

– Van Rooijen,N.和Sanders,A.(1994)。脂质体介导的巨噬细胞耗竭:作用机理,脂质体制备和应用。免疫学杂志,174(1-2),83-93。
– Van Rooijen,N.,Sanders,A.和van den Berg,TK(1996)。脂质体介导的氯膦酸盐和丙am的细胞内递送诱导的巨噬细胞凋亡。免疫学杂志,193(1),93-99。

 

现货clodronateliposomes CP-005-005说明书

现货clodronateliposomes CP-005-005说明书

上海金畔生物clodronateliposomes CP-005-005说明书

CLODRONATE脂质体和对照脂质体(PBS)

SKU: CP-005-005

类别:脂质体

描述

脂质体制剂

Clodronate脂质体:

人造球体由同心磷脂双层组成,其包封含有氯膦酸盐的含水PBS(磷酸盐缓冲盐水)溶液。脂质体是颗粒,大小大。3微米。它们在PBS中形成悬浮液,并且在储存时倾向于沉淀,在管或烧瓶的底部形成沉淀。在给予实验动物之前应该摇动悬浮液以回收均匀的悬浮液。

对照脂质体(PBS):

对照脂质体不含氯膦酸盐。仅含有PBS,它们可用于对照实验,以确定注射氯膦酸盐脂质体后观察到的效果是否仅由于巨噬细胞耗竭。

clodronateliposomes CP-005-005说明书

clodronateliposomes CP-005-005说明书
用于存储、使用和管理
抵达后存放
√到达后冷藏(4-8°C/39-46°F)不要冷冻脂质体。
√不要将脂质体加热超过40°摄氏度(104°F)。√荧光标记脂质体:保持避光!
√我们建议我们的客户在小瓶上提到的过期日期之前使用我们的产品。
使用说明
脂质体小瓶含有脂质体的悬浮液(大小为150nm-3微米)在磷酸盐缓冲盐水(PBS)中)。在这种悬浮液中,脂质体倾向于稳定下来。脂质体应在使用前摇动,以确保均匀悬浮。确保脂质体至少在室温下,消毒小瓶帽并使用无菌针。如果需要稀释悬浮液,请使用PBS或生理盐水。
行政管理

·用于静脉注射,ca。每10克动物体重应注射0.1毫升的悬浮液。(一只年轻的成年老鼠重约20
克,ca.0.2毫升)。在ca后肝脏和脾巨噬细胞的耗竭*。24小时。
腹腔注射时,脂质体通过淋巴流到达循环
因此,(“胸导管”)和耗竭将需要3天。

脂质体含对照 Clodronate Liposomes and PBS Liposomes