核紫 DCS1 死细胞染料

	货号	17549	存储条件	在零下15度以下保存, 避免光照
	规格	0.5 ml	价格	1164
	Ex (nm)	371	Em (nm)	454
	分子量	744.77	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17549

产品名称：核紫 DCS1 死细胞染料

规格：0.5ml

储存条件：-20℃干燥

产品物理化学光谱特性

溶剂：DMSO

激发波长(nm)：371

发射波长(nm)：454

产品介绍

我们的Nuclear Violet DCS1是一种非荧光，DNA选择性和细胞不透性的紫色荧光染料，用于分析死细胞中的DNA含量。 核紫色DCS1在与双链DNA结合后具有明显增强的荧光。 它可用于荧光成像，微孔板和流式细胞仪应用。 这种DNA结合染料可用于死细胞的多色分析。

点击查看光谱

产品说明书

染色细胞分析方案

注意：以下方案适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

工作溶液配制

使用缓冲液将核紫 DCS1储备溶液（5mM）稀释到0.5-5μm的浓度。

操作步骤

1.将Nuclear Violet DCS1工作溶液加到固定、死亡或凋亡的细胞（悬浮液或粘附细胞）中，并将细胞染色15至60分钟。 在最初的实验中，最好尝试几种染料浓度来确定产生所需结果的最佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。

2.用荧光显微镜、荧光酶标仪或流式细胞仪直接检测染色细胞。

图示

图1.在96孔板上染色的固定和活（非固定）Hela细胞，与核蓝 DCS1 1μm孵育20分钟并在DAPI通道成像。

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