上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F314250 250ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F314250 250ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 250ml |
瓶口尺寸 | 24/40 |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F314250 250ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F314250 250ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 250ml |
瓶口尺寸 | 24/40 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 6081 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 100 umoles | 价格 | 0 | |
Ex (nm) | 526 | Em (nm) | 543 | |
分子量 | 1023.38 | 溶剂 | MeCN | |
产品详细介绍 |
简要概述
产品基本信息
货号:6081
产品名称:VIC亚磷酰胺
规格:100umoles
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:1023.38
溶剂:MeCN
产品介绍
VIC是一种荧光染料,初由Applied Biosystems(现为Thermofisher)开发。VIC的光谱为可见光谱的绿黄色部分。它用于在5’末端荧光标记寡核苷酸(用作各种实时PCR,杂交和基于荧光的遗传分析应用中的探针)。VIC亚磷酰胺是将VIC标签掺入寡核苷酸中方便的产品。金畔生物是AAT Bioquest的中国代理商,为您提供优质的VIC亚磷酰胺。
点击查看光谱
参考文献
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction.
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Detection and quantitation of akabane and aino viruses by multiplex real-time reverse-transcriptase PCR.
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | N-Acetyl, ACTH (1-17), human |
编码 | |
别名 | N-Acetyl, ACTH (1-17), human |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Ac-SYSMEHFRWGKPVGKKR |
序列(三字母缩写) | Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-OH (trifluoroacetate salt) |
基本描述 | |
溶解度 | |
分子量 | 2135.5 |
化学式 | C97H147N29O24S1 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F319250 250ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F319250 250ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 250ml |
瓶口尺寸 | 19/22 |
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | N-Acetyl, alpha-TGF (34-43), Methyl Ester, |
编码 | |
别名 | N-Acetyl, alpha-TGF (34-43), Methyl Ester, |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Ac-CHSGYVGVRC-Ome (Cys1 and 10 bridge) |
序列(三字母缩写) | Ac-Cys-His-Ser-Gly-Tyr-Val-Gly-Val-Arg-Cys-OMe (trifluoroacetate salt)(Cys1 and 10 bridge) |
基本描述 | |
溶解度 | |
分子量 | 1151.3 |
化学式 | C47H72N15O15S2 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | Nestor et al., BBRC, 129, 226 (1985) |
C端 | |
N端 | |
化学桥 | (Cys1 and 10 bridge) |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 6082 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 1 g | 价格 | 0 | |
Ex (nm) | 526 | Em (nm) | 543 | |
分子量 | 1023.38 | 溶剂 | MeCN | |
产品详细介绍 |
简要概述
产品基本信息
货号:6082
产品名称:VIC亚磷酰胺
规格:1g
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
分子量:1023.38
溶剂:MeCN
产品介绍
VIC是一种荧光染料,初由Applied Biosystems(现为Thermofisher)开发。VIC的光谱为可见光谱的绿黄色部分。它用于在5’末端荧光标记寡核苷酸(用作各种实时PCR,杂交和基于荧光的遗传分析应用中的探针)。VIC亚磷酰胺是将VIC标签掺入寡核苷酸中方便的产品。金畔生物是AAT Bioquest的中国代理商,为您提供优质的VIC亚磷酰胺。
点击查看光谱
参考文献
Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20
Simultaneous detection of the main black aspergilli responsible for ochratoxin A (OTA) contamination in grapes by multiplex real-time polymerase chain reaction.
Authors: Selma, M V and Martínez-Culebras, P V and Elizaquível, P and Aznar, R
Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2009): 180-8
Multiplex real-time PCR for the detection and differentiation of equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4).
Authors: Diallo, Ibrahim S and Hewitson, Glen and Wright, Lucia L and Kelly, Mark A and Rodwell, Barry J and Corney, Bruce G
Journal: Veterinary microbiology (2007): 93-103
Detection and quantitation of akabane and aino viruses by multiplex real-time reverse-transcriptase PCR.
Authors: Stram, Yehuda and Kuznetzova, Larisa and Guini, Merisol and Rogel, Arie and Meirom, Ruth and Chai, Dalia and Yadin, Hagai and Brenner, Jackob
Journal: Journal of virological methods (2004): 147-54
Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.
Authors: Jarausch, J and Kadenbach, B
Journal: European journal of biochemistry (1985): 219-25
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | N-Acetyl, a-TGF (34-43), Methyl Ester, rat |
编码 | |
别名 | N-Acetyl, a-TGF (34-43), Methyl Ester, rat |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Ac-Cys-His-Ser-Gly-Tyr-Val-Gly-Val-Arg-Cys-OMe(Cys1&Cys10 bridge) |
序列(三字母缩写) | Ac-Cys-His-Ser-Gly-Tyr-Val-Gly-Val-Arg-Cys-OMe(Cys1&Cys10 bridge) |
基本描述 | |
溶解度 | |
分子量 | 1151.3 |
化学式 | C47H72N15O15S2 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | Nestor et al., BBRC, 129, 226 (1985) |
C端 | |
N端 | |
化学桥 | (Cys1&Cys10 bridge) |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F311250 250ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F311250 250ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 250ml |
瓶口尺寸 | 14/20 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 60015 | 存储条件 | 在2-8度冷藏保存, 避免光照 |
规格 | 50 mL | 价格 | 1836 |
Ex (nm) | Em (nm) | ||
分子量 | N/A | 溶剂 | Water |
产品详细介绍 |
简要概述
产品基本信息
货号:60015
产品名称:ReadiUse 病毒RNA裂解缓冲液
规格:50ml
储存条件:-15℃避光防潮
保质期:24个月
产品介绍
目前对SARS-CoV-2的RT-PCR测试需求导致了全球范围内RNA提取试剂盒的短缺。提取试剂盒制造商和医院所需的关键缺失组分是RNA裂解缓冲液。这种RediUse 病毒RNA裂解缓冲液可以用作Roche MagNA Pure 96外部裂解缓冲液(#:06374913001),BiomérieuxNuclisens裂解缓冲液(#:280134)和Qiagen Buffer RNAeasy裂解缓冲液(#:79216)的替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 病毒RNA裂解缓冲液。
参考文献
Cell Lysis Based on an Oscillating Microbubble Array.
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)
Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)
PTPN21-CDSlong isoform inhibits the response of acute lymphoblastic leukemia cells to NK-mediated lysis via the KIR/HLA-I axis.
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312
Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Authors: Pirovich, David B and Da’dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)
Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.
Authors: Rodríguez, Antonio and Vaneechoutte, Mario
Journal: BMC microbiology (2019): 94
Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1.
Authors: Bu, Shixia and Li, Boning and Wang, Qian and Gu, Tingting and Dong, Qianggang and Miao, Xiaofei and Lai, Dongmei
Journal: International journal of oncology (2019): 179-190
Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
Authors: Sun, Baojian and Bosch, Albert and Myrmel, Mette
Journal: Journal of virological methods (2019): 113638
High-resolution studies of lysis-lysogeny decision-making in bacteriophage lambda.
Authors: Shao, Qiuyan and Trinh, Jimmy T and Zeng, Lanying
Journal: The Journal of biological chemistry (2019): 3343-3349
Transcriptomic responses of the marine cyanobacterium Prochlorococcus to viral lysis products.
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028
scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling.
Authors: Dura, Burak and Choi, Jin-Young and Zhang, Kerou and Damsky, William and Thakral, Durga and Bosenberg, Marcus and Craft, Joe and Fan, Rong
Journal: Nucleic acids research (2019): e16
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | N-Acetyl, a-TGF (34-43), Methyl Ester, rat |
编码 | |
别名 | N-Acetyl, a-TGF (34-43), Methyl Ester, rat |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Ac-CHSGYVGVRC-Ome(Cys1&Cys10 bridge) |
序列(三字母缩写) | Ac-Cys-His-Ser-Gly-Tyr-Val-Gly-Val-Arg-Cys-OMe(Cys1&Cys10 bridge) |
基本描述 | [Cys2, Tyr3,Orn5,Pen7]. This somatostatin analog is a very potent and highly selective ligand for m-opioid receptors. |
溶解度 | |
分子量 | 1151.3 |
化学式 | C47H72N15O15S2 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | Nestor et al., BBRC, 129, 226 (1985) |
C端 | |
N端 | |
化学桥 | (Cys1&Cys10 bridge) |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F318200 200ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F318200 200ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 200ml |
瓶口尺寸 | 29/42 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 60016 | 存储条件 | 在2-8度冷藏保存, 避免光照 |
规格 | 500 ML | 价格 | 11988 |
Ex (nm) | Em (nm) | ||
分子量 | N/A | 溶剂 | Water |
产品详细介绍 |
简要概述
产品基本信息
货号:60016
产品名称:ReadiUse 病毒RNA裂解缓冲液
规格:500ml
储存条件:-15℃避光防潮
保质期:24个月
产品介绍
目前对SARS-CoV-2的RT-PCR测试需求导致了全球范围内RNA提取试剂盒的短缺。提取试剂盒制造商和医院所需的关键缺失组分是RNA裂解缓冲液。这种RediUse 病毒RNA裂解缓冲液可以用作Roche MagNA Pure 96外部裂解缓冲液(#:06374913001),BiomérieuxNuclisens裂解缓冲液(#:280134)和Qiagen Buffer RNAeasy裂解缓冲液(#:79216)的替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 病毒RNA裂解缓冲液。
参考文献
Cell Lysis Based on an Oscillating Microbubble Array.
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)
Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)
PTPN21-CDSlong isoform inhibits the response of acute lymphoblastic leukemia cells to NK-mediated lysis via the KIR/HLA-I axis.
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312
Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Authors: Pirovich, David B and Da’dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)
Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.
Authors: Rodríguez, Antonio and Vaneechoutte, Mario
Journal: BMC microbiology (2019): 94
Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1.
Authors: Bu, Shixia and Li, Boning and Wang, Qian and Gu, Tingting and Dong, Qianggang and Miao, Xiaofei and Lai, Dongmei
Journal: International journal of oncology (2019): 179-190
Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
Authors: Sun, Baojian and Bosch, Albert and Myrmel, Mette
Journal: Journal of virological methods (2019): 113638
High-resolution studies of lysis-lysogeny decision-making in bacteriophage lambda.
Authors: Shao, Qiuyan and Trinh, Jimmy T and Zeng, Lanying
Journal: The Journal of biological chemistry (2019): 3343-3349
Transcriptomic responses of the marine cyanobacterium Prochlorococcus to viral lysis products.
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028
scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling.
Authors: Dura, Burak and Choi, Jin-Young and Zhang, Kerou and Damsky, William and Thakral, Durga and Bosenberg, Marcus and Craft, Joe and Fan, Rong
Journal: Nucleic acids research (2019): e16
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 5506 | 存储条件 | 在2-8度冷藏保存, 避免光照 | |
规格 | 5 Labelings | 价格 | 18288 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | |||
产品详细介绍 |
简要概述
Buccutite 过氧化物酶(HRP)抗体标记试剂盒提供了一种以微观方式标记抗体的便捷方法。蛋白质 – 蛋白质缀合通常用双功能接头(例如常用的SMCC)进行,在每个末端具有不同的反应性以连接两种不同的蛋白质。交联剂的一端(通过NHS酯)与氨基酸赖氨酸和N-末端中发现的胺(-NH2)反应,另一端(通过马来酰亚胺)与氨基酸中发现的硫醇基团(-SH)反应。半胱氨酸。然而,SMCC修饰的蛋白质极不稳定并且通常是自身反应性的,因为蛋白质通常含有胺和硫醇基团,其引起显着量的均聚交联。此外,量化蛋白质上马来酰亚胺基团的数量是相当困难和繁琐的。 Buccutite 过氧化物酶(HRP)抗体偶联试剂盒设计用于直接从蛋白质,肽和含有游离氨基的其他配体制备辣根过氧化物酶(HRP)偶联物。我们的试剂盒中提供的HRP已使用我们专有的接头Buccutite FOL预先活,可直接用于缀合。 Buccutite FOL活化的HRP在极其温和的中性条件下容易与含有Buccutite MTA的分子反应,无需任何催化剂。与常用的SMCC和其他类似技术相比,我们的Buccutite 生物共轭系统更易于使用。它能够以更高的效率和产量实现生物分子的更快和定量缀合。金畔生物是AAT Bioquest的中国代理商,为您提供优质的Buccutite 过氧化物酶(HRP)抗体偶联试剂盒。
产品说明书
实验方案
操作步骤
1.运行Antibody-Buccutite MTA反应
1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。
1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。
2.制备抗体-HRP缀合物
2.1通过向Buccutite FOL活化的HRP(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的HRP溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。
2.2将整瓶Buccutite FOL活化的HRP溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。
2.3抗体-HRP结合物现在可以使用了。 注意:立即使用时,抗体-HRP结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-HRP结合物溶液。
3.抗体-HRP共轭物的储存
抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-HRP缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-HRP缀合物冻干并在≤-20℃下储存。
参考文献
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749
Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171
Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9
Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226
[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82
相关产品
产品名称 | 货号 |
Buccutite 过氧化物酶(HRP)抗体偶联试剂盒 适合标记25ug蛋白 | Cat#5505 |
Buccutite 过氧化物酶(HRP)抗体偶联试剂盒 适合标记1mg蛋白 | Cat#5504 |
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | Nafarelin |
编码 | [76932-56-4] |
别名 | Nafarelin |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Pyr-HWSY-D-2-Nal-LRPG-NH2 |
序列(三字母缩写) | Pyr-His-Trp-Ser-Tyr-D-2-Nal-Leu-Arg-Pro-Gly-NH2 |
基本描述 | Goserelin acetate, a synthetic peptide analog of LHRH, is used in the treatment of prostate cancer, advanced breast cancer and in the management of endometriosis. |
溶解度 | |
分子量 | 1322.49 |
化学式 | C66H83N17O13 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | P.Chrisp and K.L.Goa, Drugs, 39, 523 (1990) T.Mizutani et al., Int. J. Fertil. Menopaus. Stud., 40, 106 (1995) Merck Index, 14th ed., No. 6351, (2006) |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F314200 200ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F314200 200ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 200ml |
瓶口尺寸 | 24/40 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 1965 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 2 Labelings | 价格 | 2472 | |
Ex (nm) | 757 | Em (nm) | 779 | |
分子量 | 溶剂 | |||
产品详细介绍 |
简要概述
ReadiLink™xtra快速抗体标记试剂是AAT 研发,由金畔代理销售的iFluor750标记的抗体标记试剂盒。ReadiLink™xtra快速抗体标记试剂盒基本上只需要2个简单的混合步骤,而无需纯化。ReadiLink™试剂盒中使用的预活化iFluor™750非常稳定,对抗体表现出良好的反应性和选择性。该试剂盒具有用于标记〜2×50 ug抗体的所有基本成分。试剂盒中提供的两个预活化iFluor™750染料小瓶中的每一个均经过优化,可标记约50 µg抗体。ReadiLink™xtra iFluor™750快速抗体标记试剂盒提供了一种方便而强大的方法,可以使用近红外荧光的iFluor™750荧光团标记单克隆和多克隆抗体。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的抗体标记试剂盒。
点击查看光谱
产品说明书
实验方案
工作溶液配制
为了标记50 µg蛋白质(假设目标蛋白质浓度为1 mg / mL),请将5 µL(反应总体积的10%)反应缓冲液(组分B)与50 µL目标蛋白质溶液混合。
注意:如果蛋白质浓度不同,请相应地调整蛋白质体积,以使〜50 µg蛋白质可用于标记反应。
注意:要标记100 µg蛋白质(假设目标蛋白质浓度为1 mg / mL),请将10 µL(反应总体积的10%)反应缓冲液(组分B)与100 µL目标蛋白质溶液混合。
注意:蛋白质应溶于1X磷酸盐缓冲盐水(PBS),pH 7.2-7.4;如果蛋白质溶解在甘氨酸缓冲液中,则必须针对1X PBS(pH 7.2-7.4)进行透析,或使用10 kDa的Amicon Ultra-0.5,Ultracel-10膜去除游离的胺或铵盐(例如硫酸铵和乙酸铵)被广泛用于蛋白质沉淀。
注意:为获得最佳标记效率,建议最终蛋白质浓度范围为1-2 mg / mL,结合效率明显降低,低于1 mg / mL。
操作步骤
蛋白质缀合物应在载体蛋白(例如0.1%牛血清白蛋白)存在下以> 0.5 mg / mL的浓度存储。为了更长的存储时间,可以将蛋白质缀合物冻干或分成单份使用,并存储在≤–20°C下。
图示
图1.将 HL-60细胞与(红色)或没有(绿色)抗人HLA-ABC(W6 / 32 mAb)一起孵育。然后将细胞与使用ReadiLink™xtra Rapid iFluor™750抗体标记试剂盒(#1965)标记的山羊抗小鼠IgG一起孵育。使用APC-Cy7通道中的ACEA NovoCyte流式细胞仪检测荧光信号。
参考文献
Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
Authors: Sun, Weiping and Huo, Yinbo and Mei, Yuxuan and Zhou, Qingtong and Zhao, Suwen and Zhuang, Min
Journal: ACS chemical biology (2020): 39-43
Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
Authors: Noviana, Eka and Jain, Sidhartha and Hofstetter, Josephine and Geiss, Brian J and Dandy, David S and Henry, Charles S
Journal: Analytical and bioanalytical chemistry (2020): 3051-3061
A neuraminidase potency assay for quantitative assessment of neuraminidase in influenza vaccines.
Authors: Byrne-Nash, Rose T and Gillis, Jacob H and Miller, David F and Bueter, Katie M and Kuck, Laura R and Rowlen, Kathy L
Journal: NPJ vaccines (2019): 3
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.
Authors: Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald
Journal: Molecular imaging and biology (2019): 54-66
Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes.
Authors: Gao, Fenglei and Fan, Taotao and Ou, Shanshan and Wu, Jing and Zhang, Xing and Luo, Jianjun and Li, Na and Yao, Yao and Mou, Yingfeng and Liao, Xianjiu and Geng, Deqin
Journal: Biosensors & bioelectronics (2018): 201-208
Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Authors: Lu, Xuewen and Mei, Ting and Guo, Qi and Zhou, Wenjing and Li, Xiaomei and Chen, Jitao and Zhou, Xinke and Sun, Ning and Fang, Zhiyuan
Journal: Mikrochimica acta (2018): 2
Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
Authors: Stephen, Laurie and Schwarz, Emanuel and Guest, Paul C
Journal: Advances in experimental medicine and biology (2017): 149-156
Multiplex Immunoassay Profiling.
Authors: Stephen, Laurie
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 169-176
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F319200 200ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F319200 200ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 200ml |
瓶口尺寸 | 19/22 |
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | NapSul-Ile-Trp-CHO |
编码 | |
别名 | NapSul-Ile-Trp-CHO |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | NapSul-IW-CHO |
序列(三字母缩写) | NapSul-Ile-Trp-CHO |
基本描述 | Fluorogenic peptide used in the analysis of chymotrypsin-like activity of trypsins in addition to chymase from rat mast cells. Furthermore, it is a substrate for a chymotrpsin-like enzyme from sperm extract of the ascidian Halocynthia roretzi and a strong inhibitor of fertilization. It is also a substrate for carboxypeptidase Y, proteinase yscE, porcine calpain isozymes I and II, papain, and the multicatalytic proteinase, ingensin. |
溶解度 | |
分子量 | 491.6 |
化学式 | |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | V101060 | 存储条件 | Multiple |
规格 | 96 Tests | 价格 | 11988 |
Ex (nm) | Em (nm) | ||
分子量 | 溶剂 | ||
产品详细介绍 |
简要概述
Mabtech的经过仔细验证的ELISAPRO试剂盒提供了所有必需的试剂,以方便,可靠,特异的方式方便地定量测定血清,血浆和细胞培养上清液中的分析物。
ELISAPRO试剂盒随附有预先涂有单克隆抗体(mAb)。样品中的分析物被包被的mAb捕获,并被生物素化的检测mAb和链霉亲和素-HRP(SA-HRP)检测。注:TMB底物的添加将导致底物出现颜色。用硫酸终止反应,并且可以使用ELISA板酶标仪定量光密度。通过与平行分析的ELISA标准品的系列稀释液比较来确定分析物的浓度。
ELISAPRO试剂盒包括Apo ELISA缓冲液,一种可防止假阳性信号的缓冲液。缓冲液可阻止嗜异性抗体交联测定抗体。嗜异性抗体通常存在于人血清/血浆中,也可以存在于其他物种中。缓冲液已使用来自健康人类献血者的血清/血浆样品进行了验证。
Stop溶液0.18 M H2SO4(<1%)对眼睛和皮肤有刺激性,应小心处理。由于不知道暴露的影响,因此应仔细处理该标准。溶液中的缓冲液和试剂含有防腐剂Kathon CG(0.002%),这是一种潜在的过敏原,可能通过皮肤接触引起过敏。人和动物样品应被视为潜在危险的生物材料。所有材料应按照当地法规进行处理。有关更多信息,请查阅我们网站上的《安全数据表》。
在开始测定之前,让板和测定试剂达到室温(TMB底物除外,最好使用冷底物)。
计划板布局,使其一式两份,包括标准曲线,样品和测定背景对照。每孔的体积不应超过100μl。
产品说明书
样品实验方案
储备溶液配制
将50 ml洗涤缓冲液浓缩液添加到950 ml蒸馏水或去离子水中(足以完成1个板的所有洗涤步骤)。如果在20倍浓缩物中形成了晶体,则将其升至室温并轻轻混合以溶解。
用蒸馏水或去离子水将Apo ELISA缓冲液浓缩液稀释5倍,以制备所需体积的Apo ELISA缓冲液。对于每个板,将30 ml Apo ELISA缓冲液浓缩液添加到120 ml水中。
所有样品均应在Apo ELISA缓冲液中稀释至少2倍。除去可见的沉淀物并在聚丙烯管/板中稀释,应在样品之前添加缓冲液。高度溶血和高血脂的样品可能会导致定量结果不准确。为防止不同的LDL粒径干扰,所有血清/血浆来源的样品均应在Triton X-100缓冲液中稀释2倍,然后涡旋振荡5秒钟。Triton X稀释对于细胞系产生的样品不是必需的,并且不会干扰其他载脂蛋白的分析。避免冻融循环,因为它会导致信号降低。
根据对空腹健康受试者的反复分析,我们建议稀释系数为5000X。精确的移液非常重要,在稀释步骤之间更换吸头,并使用新鲜制成的稀释液。试剂的数量足以重复使用。
apoB标准品是由50%甘油稳定的纯化LDL。浓度为125μg/ ml。无需分装标准液,因为高含量的甘油可使标准液保持液态。储存在-20°C。
如图所示,稀释标准储备溶液以创建标准曲线。试剂的数量足以重复使用。最后一个样品瓶用作测定背景对照,即应省略标准液。在使用30分钟内准备标准曲线。
使用后15分钟内,将检测抗体在Apo ELISA缓冲液中稀释至1μg/ ml的浓度。对于每个板,在12 ml Apo ELISA缓冲液中稀释12μl检测抗体。
在使用后的15分钟内,将抗生蛋白链菌素-HRP用抗生蛋白链菌素-HRP稀释剂稀释1000倍。对于每块板,在12 ml的链霉亲和素-HRP稀释液中稀释12μl的链霉亲和素-HRP。
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F311200 200ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F311200 200ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 200ml |
瓶口尺寸 | 14/20 |