上海金畔生物科技有限公司提供厚壁鸡心磨口烧瓶,欣维尔,F329205L 5ml,可以访问官网了解更多产品信息。
厚壁鸡心磨口烧瓶,欣维尔,F329205L 5ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。应广大老师要求,14/20 和19/22 磨口鸡心瓶都加长了15mm 的瓶颈(24/40 磨口为无脖),易于三爪夹的固定。
技术参数:
材质 | 玻璃 |
规格(mL) | 5ml |
瓶口尺寸 | 19/22 |
上海金畔生物科技有限公司提供厚壁鸡心磨口烧瓶,欣维尔,F329205L 5ml,可以访问官网了解更多产品信息。
厚壁鸡心磨口烧瓶,欣维尔,F329205L 5ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。应广大老师要求,14/20 和19/22 磨口鸡心瓶都加长了15mm 的瓶颈(24/40 磨口为无脖),易于三爪夹的固定。
技术参数:
材质 | 玻璃 |
规格(mL) | 5ml |
瓶口尺寸 | 19/22 |
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | MMP-3 Substrate I, Fluorogenic |
编码 | |
别名 | MMP-3 Substrate I, Fluorogenic |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | DNP-PYAYWMR |
序列(三字母缩写) | DNP-Pro-Tyr-Ala-Tyr-Trp-Met-Arg-OH (trifluoroacetate salt) |
基本描述 | Best fluorogenic substrate developed for matrilysin (also called punctuated metalloproteinase 1, PUMP-1 or MMP-7) thus far. Besides good kinetic parameters (kcat/km= 1.9 x 105?M-1s-1), it presents good solubility in assay buffer. |
溶解度 | |
分子量 | 0 |
化学式 | C54H65N13O14S1 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | S. Netzel-Arnett, et al., Anal. Biochem. 195, 86 (1991) |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 1792 | 存储条件 | 在零下15度以下保存, 避免光照 |
规格 | 5 mg | 价格 | 3732 |
Ex (nm) | 579 | Em (nm) | 603 |
分子量 | 807.74 | 溶剂 | DMSO |
产品详细介绍 |
简要概述
产品基本信息
货号:1792
产品名称:Alpha Fluor 568 酸
规格:5mg
储存条件:保存在冰箱-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:807.74
外观:固体
溶剂:DMSO
激发波长(nm):579
发射波长(nm):603
产品介绍
Alpha Fluor 568酸与AlexaFluor®568酸是同一分子(AlexaFluor®是ThermoFisher的商标)。它是一种亮红色荧光染料,最适合与568 nm激光线一起使用。Alpha Fluor 568染料是水溶性的,并且在pH 4至pH 10范围内对pH值不敏感。AlphaFluor 568是最经济的胺反应形式,可将AlexaFluor®568荧光团缀合到肽上。
点击查看光谱
参考文献
A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy
Authors: Fan, S., Webb, J. E. A., Yang, Y., Nieves, D. J., Goncales, V. R., Tran, J., Hilzenrat, G., Kahram, M., Tilley, R. D., Gaus, K., Gooding, J. J.
Journal: Angew Chem Int Ed Engl (2019): 14495-14498
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates
Authors: Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugl and RP.
Journal: J Histochem Cytochem (2003): 1699
相关产品
产品名称 | 货号 |
AF 488叠氮化钠 等同于Alexa Fluor 488 | Cat#1701 |
AF 488胺 等同于Alexa Fluor 488 | Cat#1705 |
荧光标记染料 Alpha Fluor 488酸 相当于Alexa Fluor 488酸 | Cat#1790 |
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | MMP-7 Substrate (Matrilysin Substrate, PUMP-1 Substrate), Fluorogenic |
编码 | |
别名 | MMP-7 Substrate (Matrilysin Substrate, PUMP-1 Substrate), Fluorogenic |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | DNP-RPLALWRS |
序列(三字母缩写) | DNP-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser-OH (trifluoroacetate salt) |
基本描述 | Fluorogenic substrate for human neutrophil collagenase (HNC; MMP-8). |
溶解度 | |
分子量 | 0 |
化学式 | C52H77N17O14 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | A.R. Welch, et al., Arch. Biochem. Biophys. 324, 59 (1995) |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供厚壁鸡心磨口烧瓶,欣维尔,F321405L 5ml,可以访问官网了解更多产品信息。
厚壁鸡心磨口烧瓶,欣维尔,F321405L 5ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。应广大老师要求,14/20 和19/22 磨口鸡心瓶都加长了15mm 的瓶颈(24/40 磨口为无脖),易于三爪夹的固定。
技术参数:
材质 | 玻璃 |
规格(mL) | 5ml |
瓶口尺寸 | 14/20 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 5260 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 50 mg | 价格 | 6252 | |
Ex (nm) | Em (nm) | |||
分子量 | 1032.23 | 溶剂 | DMF | |
产品详细介绍 |
简要概述
产品基本信息
货号:5260
产品名称:QSY-21标记的Lys赖氨酸
规格:50mg
储存条件:保存在冰箱-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:1032.23
外观:固体
溶剂:DMF
激发波长(nm):N/A
发射波长(nm):N/A
产品介绍
FMOC-Lys(QSY-21)-OH是通过QSY-21进行序列Lys标记的基础。QSY-21是一种淬灭剂,可用于开发Cy5或其他具有类似(Cy5)荧光光谱的荧光染料(例如TF5或iFluor 647染料)与FRET探针。
点击查看光谱
参考文献
A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide.
Authors: Ji, Ao and Fan, Yichong and Ren, Wei and Zhang, Shen and Ai, Hui-Wang
Journal: ACS sensors (2018): 992-997
Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy.
Authors: Szymanski, Craig J and Yi, Hong and Liu, Joshua L and Wright, Elizabeth R and Payne, Christine K
Journal: Methods in molecular biology (Clifton, N.J.) (2013): 21-33
Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice.
Authors: de la Zerda, Adam and Bodapati, Sunil and Teed, Robert and May, Salomón Y and Tabakman, Scott M and Liu, Zhuang and Khuri-Yakub, Butrus T and Chen, Xiaoyuan and Dai, Hongjie and Gambhir, Sanjiv S
Journal: ACS nano (2012): 4694-701
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells.
Authors: Takahashi, Shodai and Piao, Wen and Matsumura, Yuriko and Komatsu, Toru and Ueno, Tasuku and Terai, Takuya and Kamachi, Toshiaki and Kohno, Masahiro and Nagano, Tetsuo and Hanaoka, Kenjiro
Journal: Journal of the American Chemical Society (2012): 19588-91
A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics.
Authors: Kabelác, Martin and Zimandl, Filip and Fessl, Tomás and Chval, Zdenek and Lankas, Filip
Journal: Physical chemistry chemical physics : PCCP (2010): 9677-84
Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
Authors: Jablonski, Amy E and Kawakami, Takashi and Ting, Alice Y and Payne, Christine K
Journal: The journal of physical chemistry letters (2010): 1312-1315
An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging.
Authors: Maxwell, Dustin and Chang, Qing and Zhang, Xu and Barnett, Edward M and Piwnica-Worms, David
Journal: Bioconjugate chemistry (2009): 702-9
Dissolved core alginate microspheres as “smart-tattoo” glucose sensors.
Authors: Chaudhary, Ayesha and Raina, Monica and McShane, Michael J and Srivastava, Rohit
Journal: Conference proceedings : … Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference (2009): 4098-101
Evaluation of glucose sensitive affinity binding assay entrapped in fluorescent dissolved-core alginate microspheres.
Authors: Chaudhary, Ayesha and Raina, Monica and Harma, Harri and Hanninen, Pekka and McShane, Michael J and Srivastava, Rohit
Journal: Biotechnology and bioengineering (2009): 1075-85
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin “quencher-chaser”: a dual “quench and chase” strategy to improve target to nontarget ratios for molecular imaging of cancer.
Authors: Ogawa, Mikako and Kosaka, Nobuyuki and Choyke, Peter L and Kobayashi, Hisataka
Journal: Bioconjugate chemistry (2009): 147-54
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | MMP-7 Substrate I, fluorogenic |
编码 | |
别名 | MMP-7 Substrate I, fluorogenic |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | DNPRPLALWRS |
序列(三字母缩写) | Dnp-Arg-Pro-Leu-Ala-Leu-Trp-Arg-Ser |
基本描述 | |
溶解度 | |
分子量 | 1164.31 |
化学式 | C52H77N17O14 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供具温度计套管磨口鸡心瓶,欣维尔,200ml F139200,可以访问官网了解更多产品信息。
具温度计套管磨口鸡心瓶,欣维尔,200ml F139200
产品简介:侧边螺纹口可插入5mm 至7.5mmOD 的普通温度计。
技术参数:
材质 | 玻璃 |
规格(mL) | 200ml |
瓶口尺寸 | 19/22 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17658 | 存储条件 | Multiple |
规格 | 100 Tests | 价格 | 1200 |
Ex (nm) | 509 | Em (nm) | 529 |
分子量 | 溶剂 | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17658
产品名称:Portelite 荧光法RNA定量试剂盒
规格:100 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
成分
成分A:StrandBrite 绿色 | 0.25 mL(在DMSO中为200X) |
成分B:测定缓冲液 | 1瓶(50ml) |
成分C:RNA标准#1 | 1 mL(RNA:0 ng / µL) |
成分D:RNA标准#2 | 4 X 250 µL(RNA:10 ng / µL) |
适用仪器
Qubit 荧光计 | |
激发: | 480nm |
发射: | 530nm |
器材: | 0.2 mL PCR小瓶 |
CytoCite 荧光计 | |
激发: | 480nm |
发射: | 530nm |
推荐孔板: | 0.2 mL PCR小瓶 |
产品介绍
检测和定量少量RNA对于多种生物学应用极为重要,例如在进行Northern blot分析,S1核酸酶测定,RNase保护测定,cDNA文库制备,反转录之前,测量体外转录RNA的产量和测定RNA浓度。 PCR和差异显示PCR。测量核酸浓度最常用的技术是测定260 nm的吸光度。基于吸收率的方法受到蛋白质,游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。敏感和选择性荧光核酸染色剂的使用减轻了这种干扰问题。StrandBrite RNA定量试剂是一种超灵敏的荧光核酸染料,用于定量溶液中的RNA。使用CytoCite 或Qubit 荧光仪,StrandBrite RNA定量试剂可检测低至5 ng / mL的RNA。我们的StrandBrite Green荧光RNA定量试剂盒包括我们的StrandBrite Green核酸染色剂,稳定性极高。它提供了一种定量溶液中RNA的简便方法。
点击查看光谱
参考文献
Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots.
Authors: Weber, Jenna and Sahoo, Malaya K and Taylor, Nathaniel and Shi, Run-Zhang and Pinsky, Benjamin A
Journal: Journal of clinical microbiology (2019)
Oncologic Implications of Chronic Hepatitis C Virus Infection.
Authors: Hwang, Jessica P and LoConte, Noelle K and Rice, John P and Foxhall, Lewis E and Sturgis, Erich M and Merrill, Janette K and Torres, Harrys A and Bailey, Howard H
Journal: Journal of oncology practice (2019): 629-637
Outcomes and costs of single-step hepatitis C testing in primary care, Birmingham, United Kingdom.
Authors: Munang, M and Smit, E and Barnett, T and Atherton, C and Tahir, M and Atabani, S F
Journal: Public health (2019): 40-44
PARP-1/2 Inhibitor Olaparib Prevents or Partially Reverts EMT Induced by TGF-β in NMuMG Cells.
Authors: Schacke, Michelle and Kumar, Janani and Colwell, Nicholas and Hermanson, Kole and Folle, Gustavo A and Nechaev, Sergei and Dhasarathy, Archana and Lafon-Hughes, Laura
Journal: International journal of molecular sciences (2019)
Tumor-draining lymph nodes demonstrate a suppressive immunophenotype in patients with non-small cell lung cancer assessed by endobronchial ultrasound-guided transbronchial needle aspiration: A pilot study.
Authors: Murthy, Vivek and Katzman, Daniel P and Tsay, Jun-Chieh J and Bessich, Jamie L and Michaud, Gaetane C and Rafeq, Samaan and Minehart, Janna and Mangalick, Keshav and de Lafaille, M A Curotto and Goparaju, Chandra and Pass, Harvey and Sterman, Daniel H
Journal: Lung cancer (Amsterdam, Netherlands) (2019): 94-99
A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection.
Authors: Huang, Chung-Guei and Lee, Li-Ang and Wu, Yi-Cheng and Hsiao, Mei-Jen and Horng, Jim-Tong and Kuo, Rei-Lin and Huang, Chih-Heng and Lin, Ya-Chu and Tsao, Kuo-Chien and Chen, Min-Chi and Chen, Tse-Ching and Shih, Shin-Ru
Journal: Oncotarget (2018): 14492-14508
Comparison of the Aptima HIV-1 Quant Dx assay with the COBAS AmpliPrep/COBAS TaqMan HIV-1 v2.0 Test for HIV-1 viral load quantification in plasma samples from HIV-1-infected patients.
Authors: Longo, Serena and Bon, Isabella and Musumeci, Giuseppina and Bertoldi, Alessia and D’Urbano, Vanessa and Calza, Leonardo and Re, Maria Carla
Journal: Health science reports (2018): e31
Evaluation of dried blood spot as an alternative sample collection method for hepatitis C virus RNA quantitation and genotyping using a commercial system.
Authors: Mahajan, Supriya and Choudhary, Manish Chandra and Kumar, Guresh and Gupta, Ekta
Journal: Virusdisease (2018): 141-146
Evaluation of two RNA extraction methods for human parechovirus detection on pediatric stool specimens.
Authors: Bergallo, Massimiliano and Montanari, Paola and Daprà, Valentina and Cuccu, Roberto and Bonamin, Stefano and Gabiano, Clara and Galliano, Ilaria
Journal: Minerva pediatrica (2018)
An RNA-based signature enables high specificity detection of circulating tumor cells in hepatocellular carcinoma.
Authors: Kalinich, Mark and Bhan, Irun and Kwan, Tanya T and Miyamoto, David T and Javaid, Sarah and LiCausi, Joseph A and Milner, John D and Hong, Xin and Goyal, Lipika and Sil, Srinjoy and Choz, Melissa and Ho, Uyen and Kapur, Ravi and Muzikansky, Alona and Zhang, Huidan and Weitz, David A and Sequist, Lecia V and Ryan, David P and Chung, Raymond T and Zhu, Andrew X and Isselbacher, Kurt J and Ting, David T and Toner, Mehmet and Maheswaran, Shyamala and Haber, Daniel A
Journal: Proceedings of the National Academy of Sciences of the United States of America (2017): 1123-1128
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | MMP-8 Substrate, fluorogenic |
编码 | |
别名 | MMP-8 Substrate, fluorogenic |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | DNPPLAYWAR |
序列(三字母缩写) | Dnp-Pro-Leu-Ala-Tyr-Trp-Ala-Arg |
基本描述 | |
溶解度 | |
分子量 | 1042.14 |
化学式 | C49H63N13O13 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供具温度计套管磨口鸡心瓶,欣维尔,25ml F131025,可以访问官网了解更多产品信息。
具温度计套管磨口鸡心瓶,欣维尔,25ml F131025
产品简介:侧边螺纹口可插入5mm 至7.5mmOD 的普通温度计。
技术参数:
材质 | 玻璃 |
规格(mL) | 25ml |
瓶口尺寸 | 14/20 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 17659 | 存储条件 | Multiple |
规格 | 500 Tests | 价格 | 3732 |
Ex (nm) | 509 | Em (nm) | 529 |
分子量 | 溶剂 | ||
产品详细介绍 |
简要概述
产品基本信息
货号:17659
产品名称:Portelite 荧光法RNA定量试剂盒
规格:500 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
成分
成分A:StrandBrite 绿色 | 0.25 mL(在DMSO中为200X) |
成分B:测定缓冲液 | 1瓶(50ml) |
成分C:RNA标准#1 | 1 mL(RNA:0 ng / µL) |
成分D:RNA标准#2 | 4 X 250 µL(RNA:10 ng / µL) |
适用仪器
Qubit 荧光计 | |
激发: | 480nm |
发射: | 530nm |
器材: | 0.2 mL PCR小瓶 |
CytoCite 荧光计 | |
激发: | 480nm |
发射: | 530nm |
推荐孔板: | 0.2 mL PCR小瓶 |
产品介绍
检测和定量少量RNA对于多种生物学应用极为重要,例如在进行Northern blot分析,S1核酸酶测定,RNase保护测定,cDNA文库制备,反转录之前,测量体外转录RNA的产量和测定RNA浓度。 PCR和差异显示PCR。测量核酸浓度最常用的技术是测定260 nm的吸光度。基于吸收率的方法受到蛋白质,游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。敏感和选择性荧光核酸染色剂的使用减轻了这种干扰问题。StrandBrite RNA定量试剂是一种超灵敏的荧光核酸染料,用于定量溶液中的RNA。使用CytoCite 或Qubit 荧光仪,StrandBrite RNA定量试剂可检测低至5 ng / mL的RNA。我们的StrandBrite Green荧光RNA定量试剂盒包括我们的StrandBrite Green核酸染色剂,稳定性极高。它提供了一种定量溶液中RNA的简便方法。
点击查看光谱
参考文献
Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots.
Authors: Weber, Jenna and Sahoo, Malaya K and Taylor, Nathaniel and Shi, Run-Zhang and Pinsky, Benjamin A
Journal: Journal of clinical microbiology (2019)
Oncologic Implications of Chronic Hepatitis C Virus Infection.
Authors: Hwang, Jessica P and LoConte, Noelle K and Rice, John P and Foxhall, Lewis E and Sturgis, Erich M and Merrill, Janette K and Torres, Harrys A and Bailey, Howard H
Journal: Journal of oncology practice (2019): 629-637
Outcomes and costs of single-step hepatitis C testing in primary care, Birmingham, United Kingdom.
Authors: Munang, M and Smit, E and Barnett, T and Atherton, C and Tahir, M and Atabani, S F
Journal: Public health (2019): 40-44
PARP-1/2 Inhibitor Olaparib Prevents or Partially Reverts EMT Induced by TGF-β in NMuMG Cells.
Authors: Schacke, Michelle and Kumar, Janani and Colwell, Nicholas and Hermanson, Kole and Folle, Gustavo A and Nechaev, Sergei and Dhasarathy, Archana and Lafon-Hughes, Laura
Journal: International journal of molecular sciences (2019)
Tumor-draining lymph nodes demonstrate a suppressive immunophenotype in patients with non-small cell lung cancer assessed by endobronchial ultrasound-guided transbronchial needle aspiration: A pilot study.
Authors: Murthy, Vivek and Katzman, Daniel P and Tsay, Jun-Chieh J and Bessich, Jamie L and Michaud, Gaetane C and Rafeq, Samaan and Minehart, Janna and Mangalick, Keshav and de Lafaille, M A Curotto and Goparaju, Chandra and Pass, Harvey and Sterman, Daniel H
Journal: Lung cancer (Amsterdam, Netherlands) (2019): 94-99
A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients’ responses to H7N9 infection.
Authors: Huang, Chung-Guei and Lee, Li-Ang and Wu, Yi-Cheng and Hsiao, Mei-Jen and Horng, Jim-Tong and Kuo, Rei-Lin and Huang, Chih-Heng and Lin, Ya-Chu and Tsao, Kuo-Chien and Chen, Min-Chi and Chen, Tse-Ching and Shih, Shin-Ru
Journal: Oncotarget (2018): 14492-14508
Comparison of the Aptima HIV-1 Quant Dx assay with the COBAS AmpliPrep/COBAS TaqMan HIV-1 v2.0 Test for HIV-1 viral load quantification in plasma samples from HIV-1-infected patients.
Authors: Longo, Serena and Bon, Isabella and Musumeci, Giuseppina and Bertoldi, Alessia and D’Urbano, Vanessa and Calza, Leonardo and Re, Maria Carla
Journal: Health science reports (2018): e31
Evaluation of dried blood spot as an alternative sample collection method for hepatitis C virus RNA quantitation and genotyping using a commercial system.
Authors: Mahajan, Supriya and Choudhary, Manish Chandra and Kumar, Guresh and Gupta, Ekta
Journal: Virusdisease (2018): 141-146
Evaluation of two RNA extraction methods for human parechovirus detection on pediatric stool specimens.
Authors: Bergallo, Massimiliano and Montanari, Paola and Daprà, Valentina and Cuccu, Roberto and Bonamin, Stefano and Gabiano, Clara and Galliano, Ilaria
Journal: Minerva pediatrica (2018)
An RNA-based signature enables high specificity detection of circulating tumor cells in hepatocellular carcinoma.
Authors: Kalinich, Mark and Bhan, Irun and Kwan, Tanya T and Miyamoto, David T and Javaid, Sarah and LiCausi, Joseph A and Milner, John D and Hong, Xin and Goyal, Lipika and Sil, Srinjoy and Choz, Melissa and Ho, Uyen and Kapur, Ravi and Muzikansky, Alona and Zhang, Huidan and Weitz, David A and Sequist, Lecia V and Ryan, David P and Chung, Raymond T and Zhu, Andrew X and Isselbacher, Kurt J and Ting, David T and Toner, Mehmet and Maheswaran, Shyamala and Haber, Daniel A
Journal: Proceedings of the National Academy of Sciences of the United States of America (2017): 1123-1128
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) |
编码 | |
别名 | MMP-8 Substrate, Fluorogenic (Neutrophil Collagenase Substrate) |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | Dnp-PLAYWAR |
序列(三字母缩写) | DNP-Pro-Leu-Ala-Tyr-Trp-Ala-Arg-OH (trifluoroacetate salt) |
基本描述 | Highly sensitive and selective substrate for collagenase 3 (MMP-13) with a kcat/Km value of 4.22 x 106?M-1s-1?(PH= 7.5, T = 37°C) |
溶解度 | |
分子量 | 0 |
化学式 | C49H63N13O13 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | S. Netzel-Arnett, et al., Anal. Biochem. 195, 86 (1991) |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供具温度计套管磨口鸡心瓶,欣维尔,15ml F131415,可以访问官网了解更多产品信息。
具温度计套管磨口鸡心瓶,欣维尔,15ml F131415
产品简介:侧边螺纹口可插入5mm 至7.5mmOD 的普通温度计。
技术参数:
材质 | 玻璃 |
规格(mL) | 15ml |
瓶口尺寸 | 14/20 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 22520 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 200 Tests | 价格 | 2472 | |
Ex (nm) | 518 | Em (nm) | 654 | |
分子量 | 844.83 | 溶剂 | DMSO | |
产品详细介绍 |
简要概述
产品基本信息
货号:22520
产品名称:Cell Meter BX650固定化细胞活性染料
规格:200 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:N/A
激发波长(nm):518
发射波长(nm):654
适用仪器
流式细胞仪 | |
激发: | 480nm激光 |
发射: | 695/40nm滤波片 |
滤波片: | PerCP滤波片组 |
荧光显微镜 | |
激发: | TexasRed滤波片组 |
发射: | TexasRed滤波片组 |
推荐孔板: | 黑色透明 |
产品介绍
从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter BX650固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX650固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter BX650固定化细胞活性染料经过优化,可以在488 nm的蓝色激光激发下以650 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter BX650固定化细胞活性染料。
点击查看光谱
图示
图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX650(Cat#22520)染色,然后固定在3.7%甲醛中,并通过流式细胞仪进行分析。带有PerCP通道的死细胞群体(蓝色峰)很容易与活细胞群体(红色峰)区分开,在固定之前和之后都获得了几乎相同的结果。 |
参考文献
Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells.
Authors: Pereira, Túlio Felipe and Levin, Gabriel and DeOcesano-Pereira, Carlos and Caodaglio, Amanda Schiersner and Fujita, André and Tonso, Aldo and Sogayar, Mari Cleide
Journal: BMC research notes (2020): 57
[Immune function of myeloid-derived suppressor cells and its mechanism in obstructive sleep apnea syndrome].
Authors: Chen, S W and Li, J and Xiang, B and Xu, S J and Wang, L
Journal: Zhonghua yi xue za zhi (2020): 295-300
CFDA-SE Combined with MACSiBeads™ Particles to Evaluate the Inhibitory Effect of Treg Cells in vitro.
Authors: Ren, Qingqi and Jiang, Chunlin and Liu, Jikui
Journal: Annals of clinical and laboratory science (2019): 740-747
Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.
Authors: Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar
Journal: PloS one (2019): e0221878
[Effects of skin γδ T lymphocytes on wound healing of mice through regulating proliferation and differentiation of mice epidermal cells].
Authors: Zhu, H J and Li, Y S and Wang, Y P and Hu, X H and Zhang, X R and Qiu, L and He, W F and Luo, G X
Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (2019): 298-307
Impaired Immunosuppressive Effect of Bronchoalveolar Mesenchymal Stem Cells in Hypersensitivity Pneumonitis: Preliminary Findings.
Authors: Balogh, Enikő and Nagy, Béla and Gyetvai, Ágnes and Bene, Zsolt and Hendrik, Zoltán and Jeney, Viktória and Nagy, Péter and Papp, Ágnes and Balla, József and Balla, György and Kappelmayer, János and Nagy, Béla
Journal: Cytometry. Part B, Clinical cytometry (2018): 363-368
Delineating the distinct role of AKT in mediating cell survival and proliferation induced by CD154 and IL-4/IL-21 in chronic lymphocytic leukemia.
Authors: Chapman, Elinor A and Oates, Melanie and Mohammad, Ishaque S and Davies, Barry R and Stockman, Paul K and Zhuang, Jianguo and Pettitt, Andrew R
Journal: Oncotarget (2017): 102948-102964
Human auricular chondrocytes with high proliferation rate show high production of cartilage matrix.
Authors: Ishibashi, Makiko and Hikita, Atsuhiko and Fujihara, Yuko and Takato, Tsuyoshi and Hoshi, Kazuto
Journal: Regenerative therapy (2017): 21-28
[Tumor derived IgG suppress the proliferation of T cells in cord blood].
Authors: Liu, E Y and Liu, J F and Shao, W W and Xiao, L and Li, G H and Chang, X H and Qiu, X Y
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 824-828
A comparative study of colorimetric cell proliferation assays in immune cells.
Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
Journal: Cytotechnology (2016): 1489-98
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | Moth Cytochrome C (MCC)) Fragment |
编码 | [154277-61-9] |
别名 | Moth Cytochrome C (MCC)) Fragment |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | ANERADLIAYLQQATK |
序列(三字母缩写) | H-Ala-Asn-Glu-Arg-Ala-Asp-Leu-Ile-Ala-Tyr-Leu-Gln-Gln-Ala-Thr-Lys-OH (trifluoroacetate salt) |
基本描述 | An antigenic sequence used to study the T-cell response. |
溶解度 | |
分子量 | 1805.02 |
化学式 | C78H129N23O26 |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | L.M.Spain et al., J. Immunol., 152, 1709 (1994) |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供具温度计套管磨口鸡心瓶,欣维尔,10ml F131010,可以访问官网了解更多产品信息。
具温度计套管磨口鸡心瓶,欣维尔,10ml F131010
产品简介:侧边螺纹口可插入5mm 至7.5mmOD 的普通温度计。
技术参数:
材质 | 玻璃 |
规格(mL) | 10ml |
瓶口尺寸 | 14/20 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
货号 | 20116 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 25 Tests | 价格 | 4368 | |
Ex (nm) | Em (nm) | |||
分子量 | 溶剂 | |||
产品详细介绍 |
简要概述
产品基本信息
货号:20116
产品名称:Cell Meter Caspase 8活性细胞结合检测试剂盒 红色荧光
规格:25 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
试剂盒成分
成分A:iFluor 647-LETD-FMK | 1 vial |
成分B:洗涤缓冲液 | 1瓶(100ml) |
成分C:500X Nuclear Green DCS1 | 1 vial(100 µL) |
成分D:500X Hoechst | 1 vial(100 µL) |
适用仪器
流式细胞仪 | |
激发: | 640 nm激光 |
发射: | 660/20 nm滤波片 |
滤波片: | APC滤波片组 |
荧光显微镜 | |
激发: | Cy5滤波片 |
发射: | Cy5滤波片 |
推荐孔板: | 黑色透明 |
荧光酶标仪 | |
激发: | 640nm |
发射: | 680nm |
cutoff: | 665nm |
推荐孔板: | 黑色透明 |
读取模式: | 底读模式 |
产品介绍
我们的Cell Meter 活细胞胱天蛋白酶活性测定试剂盒基于胱天蛋白酶的荧光FMK抑制剂。这些抑制剂是细胞可渗透的和无细胞毒性的。一旦进入细胞,胱天蛋白酶抑制剂就与活性胱天蛋白酶共价结合。此Cell Meter Caspase 8活性细胞结合检测试剂盒旨在通过测量活细胞中的caspase 8活化来检测细胞凋亡。它用于定量凋亡细胞中激活的caspase 8活性,或用于筛选caspase 8抑制剂。iFluor 647-LETD-FMK,红色标记试剂,可通过荧光显微镜,流式细胞仪或荧光酶标仪直接检测凋亡细胞中活化的胱天蛋白酶8。该试剂盒提供所有必需成分,并具有优化的测定方案。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter Caspase 8活性细胞结合检测试剂盒。
图示
图1.在Jurkat细胞中使用Cell Meter 活细胞Caspase 8检测试剂盒对活性caspase 8进行流式细胞分析。细胞用1μM星形孢菌素处理5小时(绿色),而未处理的细胞用作对照(红色)。星形孢菌素反应被显示为蓝色的Z-VAD-FMK(胱天蛋白酶抑制剂)抑制。将细胞与iFluor 647-LETD-FMK在室温下孵育1小时。使用带有660/20 nm滤光片(APC通道)的NovoCyte流式细胞仪测量荧光强度。 |
参考文献
A matter of life and death for caspase 8.
Authors: Willson, Joseph
Journal: Nature reviews. Molecular cell biology (2020): 63
Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28.
Authors: Müller, Ines and Strozyk, Elwira and Schindler, Sebastian and Beissert, Stefan and Oo, Htoo Zarni and Sauter, Thomas and Lucarelli, Philippe and Raeth, Sebastian and Hausser, Angelika and Al Nakouzi, Nader and Fazli, Ladan and Gleave, Martin E and Liu, He and Simon, Hans-Uwe and Walczak, Henning and Green, Douglas R and Bartek, Jiri and Daugaard, Mads and Kulms, Dagmar
Journal: Molecular cell (2020): 970-984.e7
Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.
Authors: Zhong, Benfu and Liu, Miao and Bai, Changsen and Ruan, Yuxia and Wang, Yuanyuan and Qiu, Li and Hong, Yang and Wang, Xin and Li, Lifang and Li, Binghui
Journal: Molecular therapy : the journal of the American Society of Gene Therapy (2020)
Caspase-8: The double-edged sword.
Authors: Mandal, Ranadip and Barrón, Joan Compte and Kostova, Izabela and Becker, Sven and Strebhardt, Klaus
Journal: Biochimica et biophysica acta. Reviews on cancer (2020): 188357
Chrm3 protects against acinar cell necrosis by stabilizing caspase-8 expression in severe acute pancreatitis mice model.
Authors: Huang, Ning and Murtaza, Ghulam and Wang, Lujing and Luan, Jing and Wang, Xinlei and Sun, Yumiao and Wu, Xing and Tao, Yuxi and Shi, Shuoxi and Cao, Peihua and Qiao, Yu and Han, Dong and Kou, Jiayuan and Ma, Ning and Gao, Xu
Journal: Journal of cellular biochemistry (2020): 2618-2631
Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.
Authors: Buscetta, Marco and Di Vincenzo, Serena and Miele, Monica and Badami, Ester and Pace, Elisabetta and Cipollina, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 1819-1832
Dissecting DISC regulation via pharmacological targeting of caspase-8/c-FLIPL heterodimer.
Authors: Hillert, Laura K and Ivanisenko, Nikita V and Busse, Denise and Espe, Johannes and König, Corinna and Peltek, Sergey E and Kolchanov, Nikolai A and Ivanisenko, Vladimir A and Lavrik, Inna N
Journal: Cell death and differentiation (2020)
Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.
Authors: He, Tian Tian and Zhou, Ying and Liu, Ying Li and Li, Duan You and Nie, Pin and Li, Ai Hua and Xie, Hai Xia
Journal: Cellular microbiology (2020): e13193
Expression levels of EPHB4, EFNB2 and caspase-8 are associated with clinicopathological features and progression of esophageal squamous cell cancer.
Authors: Ni, Qianzhi and Chen, Pingping and Zhu, Bing and Li, Jingjing and Xie, Dong and Ma, Xingyuan
Journal: Oncology letters (2020): 917-929
High-Concentrate Feeding to Dairy Cows Induces Apoptosis via the NOD1/Caspase-8 Pathway in Mammary Epithelial Cells.
Authors: Ul Aabdin, Zain and Cheng, Xiaoye and Dai, Hongyu and Wang, Yan and Sahito, Benazir and Roy, Animesh Chandra and Memon, Meena Arif and Shen, Xiangzhen
Journal: Genes (2020)
上海金畔生物科技有限公司可以定制不同序列多肽,可以访问官网了解更多产品信息。
名称 | Motilin (human, porcine) |
编码 | [9072-41-7] |
别名 | Motilin (human, porcine) |
纯度 | 80%,90%,95%,98%,99% |
重量 | 1mg,5mg,10mg,50mg,100mg,1g |
序列(单字母缩写) | FVPIFTYGELQRMQEKERNKGQ-OH |
序列(三字母缩写) | Phe-Val-Pro-Ile-Phe-Thr-Tyr-Gly-Glu-Leu-Gln-Arg-Met-Gln-Glu-Lys-Glu-Arg-Asn-Lys-Gly-Gln |
基本描述 | |
溶解度 | |
分子量 | 0 |
化学式 | |
存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
注释 | |
Documents | |
Figures | |
Reference | |
C端 | |
N端 | |
化学桥 |
上海金畔生物科技有限公司提供厚壁茄型瓶,欣维尔,F31292L 500ml,可以访问官网了解更多产品信息。
厚壁茄型瓶,欣维尔,F31292L 500ml
产品简介:厚壁设计,球体均用玻璃管手工吹制而成。
技术参数:
材质 | 玻璃 |
规格(mL) | 500ml |
瓶口尺寸 | 29/42 |