上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404461C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404461C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 203 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
 |
货号 | 17082 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 umoles | 价格 | 11988 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 637.19 | 溶剂 | DMF | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：17082
产品名称：2-Aminoethoxypropargyl ddTTP
规格：1 umoles
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：N/A
外观：液体
溶剂：DMF
激发波长(nm)：N/A
发射波长(nm)：N/A
产品介绍
Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的2-Aminoethoxypropargyl ddTTP。
参考文献
Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4
UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7
Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65
A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23
Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72
Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405
Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39
Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY (13-36) (canine, mouse, porcine, rat) |
| 编码 | [86895-09-2] |
| 别名 | Peptide YY (13-36) (canine, mouse, porcine, rat) |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | SPEELSRYYASLRHYLNLVTRQRY-NH2 |
| 序列（三字母缩写） | Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 |
| 基本描述 | |
| 溶解度 | |
| 分子量 | 0 |
| 化学式 | |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents | ![Peptide YY (13-36) (canine, mouse, porcine, rat) 编码 [86895-09-2]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dca457e3.png) |
| Figures | ![Peptide YY (13-36) (canine, mouse, porcine, rat) 编码 [86895-09-2]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dca9bccf.jpg) |
| Reference | |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404324C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404324C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 457 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
 |
货号 | 2585 | 存储条件 | Multiple |
| 规格 | 1 mg | 价格 | 4584 | |
| Ex (nm) | 566 | Em (nm) | 708 | |
| 分子量 | N/A | 溶剂 | ||
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：2585
产品名称：ReadiUse 预活化PE-iFluor 700 Tandem
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：N/A
溶剂：水
产品介绍
ReadiUse 预活化PE-iFluor 700 Tandem是美国AAT Bioquest生产的蛋白标记染料，PE-AlexaFluor®700是流式细胞仪中常用的一种颜色。它的主要吸收峰在565 nm，发射峰在720 nm。AAT Bioquest提供了这种预活化的PE-iFluor 700 ，以替代流行的PE-AlexaFluor®700 Tandem。与串联的PE-AlexaFluor®700相比，PE-iFluor 700更亮，FRET效率更高。它用于促进PE-AlexaFluor®700串联连接至抗体和其他蛋白质，例如链霉亲和素和其他辅助试剂。我们的预活化PE-iFluor 700 Tandem比传统繁琐SMCC的PE-AlexaFluor®700 Tandem染料具有更高的产率，并具有更好的流式细胞仪性能。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse 预活化PE-iFluor 700 Tandem。
点击查看光谱
参考文献
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239
An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots.
Authors: Samanta, Anirban and Walper, Scott A and Susumu, Kimihiro and Dwyer, Chris L and Medintz, Igor L
Journal: Nanoscale (2015): 7603-14
Multicolor detection of rare tumor cells in blood using a novel flow cytometry-based system.
Authors: Watanabe, Masaru and Uehara, Yuri and Yamashita, Namiko and Fujimura, Yuu and Nishio, Kaori and Sawada, Takeshi and Takeda, Kazuo and Koizumi, Fumiaki and Koh, Yasuhiro
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2014): 206-13
Noninvasive and quantitative assessment of in vivo fetomaternal interface angiogenesis using RGD-based fluorescence.
Authors: Keramidas, M and Lavaud, J and Sergent, F and Hoffmann, P and Brouillet, S and Feige, J-J and Coll, J-L and Alfaidy, N
Journal: BioMed research international (2014): 309082
Intraoperative near-infrared image-guided surgery for peritoneal carcinomatosis in a preclinical experimental model.
Authors: Keramidas, M and Josserand, V and Righini, C A and Wenk, C and Faure, C and Coll, J L
Journal: The British journal of surgery (2010): 737-43
Homogeneous TR-FRET high-throughput screening assay for calcium-dependent multimerization of sorcin.
Authors: Appelblom, Heidi and Nurmi, Jussi and Soukka, Tero and Pasternack, Michael and Penttilä, Kai E and Lövgren, Timo and Niemelä, Pauliina
Journal: Journal of biomolecular screening (2007): 842-8
Analysis of hollow-core photonic bandgap fibers for evanescent wave biosensing.
Authors: Sun, Jian and Chan, Chi-Chiu and Zhang, Yi-Fan and Shum, Ping
Journal: Journal of biomedical optics: 054048
相关产品
| 产品名称 | 货号 |
| ReadiUse 预活化APC-iFluor 750 Tandem | Cat#2571 |
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY (3-36) (canine, mouse, porcine, rat) |
| 编码 | [126339-09-1] |
| 别名 | Peptide YY (3-36) (canine, mouse, porcine, rat) |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | AKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-NH2 |
| 序列（三字母缩写） | Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 |
| 基本描述 | |
| 溶解度 | |
| 分子量 | 4240.7 |
| 化学式 | C190H288N54O57 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents | ![Peptide YY (3-36) (canine, mouse, porcine, rat) 编码 [126339-09-1]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dc28b8fa.png) |
| Figures | ![Peptide YY (3-36) (canine, mouse, porcine, rat) 编码 [126339-09-1]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dc2e3912.jpg) |
| Reference | |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404323C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404323C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 305 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 17200 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 5 mL | 价格 | 3732 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | Water | |
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：17200
产品名称：ReadiUse 即用型dNTP Mix溶液*10mM*
规格：5ml
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
溶剂：水
产品介绍
我们的即用型脱氧核苷酸三磷酸（dNTPs）溶液已经过广泛的检测和验证，可用于各种分子生物学应用，包括PCR，实时PCR，高保真PCR，长片段PCR，cDNA合成，RT-PCR，RCA，MDA，DNA标记，DNA测序，体外转录，siRNA合成，和RNA扩增。dNTP混合物优点在于方便和灵活。每个标准核苷酸在水中以10mM供应。该试剂通过添加核苷酸作为混合物可以减少移液步骤。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse 即用型dNTP Mix溶液。
参考文献
A novel plasma based early colorectal cancer screening assay base on methylated SDC2 and SFRP2.
Authors: Zhao, Guodong and Ma, Yong and Li, Hui and Li, Shiming and Zhu, Yun and Liu, Xiaoyu and Xiong, Shangmin and Liu, Yi and Miao, Jin and Fei, Sujuan and Zheng, Minxue and Zhao, Xiangwei
Journal: Clinica chimica acta; international journal of clinical chemistry (2020): 84-89
An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells.
Authors: Browne, Daniel J and Brady, Jamie L and Waardenberg, Ashley J and Loiseau, Claire and Doolan, Denise L
Journal: Frontiers in immunology (2020): 402
Development of a nested PCR assay for detection of Streptococcus equi subspecies equi in clinical equine specimens and comparison with a qPCR assay.
Authors: Noll, Lance W and Stoy, Colin P A and Wang, Yin and Porter, Elizabeth G and Lu, Nanyan and Liu, Xuming and Burklund, Amy and Peddireddi, Lalitha and Hanzlicek, Gregg and Henningson, Jamie and Chengappa, M M and Bai, Jianfa
Journal: Journal of microbiological methods (2020): 105887
The effects of short-term combined exercise training on telomere length in obese women: a prospective, interventional study.
Authors: Brandao, Camila Fernanda Cunha and Nonino, Carla Barbosa and de Carvalho, Flavia Giolo and Nicoletti, Carolina Ferreira and Noronha, Natalia Yumi and San Martin, Rocio and de Freitas, Ellen Cristini and Junqueira-Franco, Marcia Varella Morandi and Marchini, Julio Sergio
Journal: Sports medicine – open (2020): 5
Assessment of Xanthomonas arboricola pv. juglandis Bacterial Load in Infected Walnut Fruits by Quantitative PCR.
Authors: Martins, Leonor and Fernandes, Camila and Albuquerque, Pedro and Tavares, Fernando
Journal: Plant disease (2019): 2577-2586
Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR).
Authors: Bhatia, Shama and Matthews, Jason and Wells, Peter G
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 389-403
Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Hypoxia and the Transforming Growth Factor beta 1 (TGFβ-1) and SMADs Pathway in a Mouse Model of Cirrhosis.
Authors: Zhang, Liting and Zhou, Dan and Li, Junfeng and Yan, Xiaoming and Zhu, Jun and Xiao, Ping and Chen, Tuo and Xie, Xiaodong
Journal: Medical science monitor : international medical journal of experimental and clinical research (2019): 7182-7190
Evaluating observer bias and seasonal detection rates in amphibian pathogen eDNA collections by citizen scientists.
Authors: Julian, James T and Glenney, Gavin W and Rees, Christopher
Journal: Diseases of aquatic organisms (2019): 15-24
Expression level of CEBPA gene in acute lymphoblastic leukemia individuals.
Authors: Szmajda, Dagmara and Krygier, Adrian and Jamroziak, Krzysztof and Żebrowska-Nawrocka, Marta and Balcerczak, Ewa
Journal: Scientific reports (2019): 15640
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.
Authors: Bak, Aurélie and Emerson, Joanne B
Journal: BMC biotechnology (2019): 73
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY(3-36), PYY, human |
| 编码 | [123583-37-9] |
| 别名 | Peptide YY(3-36), PYY, human |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | IKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-NH2 |
| 序列（三字母缩写） | H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2?(trifluoroacetate salt) |
| 基本描述 | This is amino acids 340 to 349 fragment of Chk-1 protein, phosphorylated at Ser345. Chk1, an evolutionarily conserved protein kinase, has been implicated in cell cycle checkpoint control in lower eukaryotes in response to DNA damage and replicative stress. CHK1 deficiency results in a severe proliferation defect and death in embryonic stem cells, and peri-implantation embryonic lethality in mice. |
| 溶解度 | |
| 分子量 | 4049.6 |
| 化学式 | C180H279N53O54 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents | ![Peptide YY(3-36), PYY, human 编码 [123583-37-9]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dba9ed67.png) |
| Figures | ![Peptide YY(3-36), PYY, human 编码 [123583-37-9]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2dbb066d3.jpg) |
| Reference | D. Grandt et al., Regulatory Peptides, 40, 161(1992) |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
 |
货号 | 1970 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 2 Labelings | 价格 | 2472 | |
| Ex (nm) | 491 | Em (nm) | 516 | |
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
ReadiLink™xtra快速抗体标记试剂是AAT 研发，由金畔代理销售的FITC标记的抗体标记试剂盒。ReadiLink™xtra快速抗体标记试剂盒基本上只需要2个简单的混合步骤，而无需纯化。ReadiLink™试剂盒中使用的预活化FITC非常稳定，对抗体表现出良好的反应性和选择性。该试剂盒具有用于标记〜2×50 ug抗体的所有基本成分。试剂盒中提供的两个预活化iFluor™488染料小瓶中的每一个均经过优化，可标记约50 µg抗体。ReadiLink™xtra FITC快速抗体标记试剂盒提供了一种方便而强大的方法，可以使用绿色的FITC荧光团标记单克隆和多克隆抗体。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的抗体标记试剂盒。
点击查看光谱
产品说明书
实验方案
工作溶液配制
为了标记50 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将5 µL（反应总体积的10％）反应缓冲液（组分B）与50 µL目标蛋白质溶液混合。
注意：如果蛋白质浓度不同，请相应地调整蛋白质体积，以使〜50 µg蛋白质可用于标记反应。
注意：要标记100 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将10 µL（反应总体积的10％）反应缓冲液（组分B）与100 µL目标蛋白质溶液混合。
注意：蛋白质应溶于1X磷酸盐缓冲盐水（PBS），pH 7.2-7.4；如果蛋白质溶解在甘氨酸缓冲液中，则必须针对1X PBS（pH 7.2-7.4）进行透析，或使用10 kDa的Amicon Ultra-0.5，Ultracel-10膜去除游离的胺或铵盐（例如硫酸铵和乙酸铵）被广泛用于蛋白质沉淀。
注意：为获得最佳标记效率，建议最终蛋白质浓度范围为1-2 mg / mL，结合效率明显降低，低于1 mg / mL。
操作步骤
蛋白质缀合物应在载体蛋白（例如0.1％牛血清白蛋白）存在下以> 0.5 mg / mL的浓度存储。为了更长的存储时间，可以将蛋白质缀合物冻干或分成单份使用，并存储在≤–20°C下。
图示
图1. HeLa细胞中微管蛋白的免疫荧光染色。HeLa细胞用4％PFA固定，用0.1％Triton X-100透化并封闭。然后将细胞与小鼠抗微管蛋白抗体一起孵育，并用ReadiLink™xtra Rapid FITC抗体标记试剂盒（#1970）标记的山羊抗小鼠IgG染色。
参考文献
Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
Authors: Sun, Weiping and Huo, Yinbo and Mei, Yuxuan and Zhou, Qingtong and Zhao, Suwen and Zhuang, Min
Journal: ACS chemical biology (2020): 39-43
Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
Authors: Noviana, Eka and Jain, Sidhartha and Hofstetter, Josephine and Geiss, Brian J and Dandy, David S and Henry, Charles S
Journal: Analytical and bioanalytical chemistry (2020): 3051-3061
A neuraminidase potency assay for quantitative assessment of neuraminidase in influenza vaccines.
Authors: Byrne-Nash, Rose T and Gillis, Jacob H and Miller, David F and Bueter, Katie M and Kuck, Laura R and Rowlen, Kathy L
Journal: NPJ vaccines (2019): 3
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.
Authors: Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald
Journal: Molecular imaging and biology (2019): 54-66
Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes.
Authors: Gao, Fenglei and Fan, Taotao and Ou, Shanshan and Wu, Jing and Zhang, Xing and Luo, Jianjun and Li, Na and Yao, Yao and Mou, Yingfeng and Liao, Xianjiu and Geng, Deqin
Journal: Biosensors & bioelectronics (2018): 201-208
Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Authors: Lu, Xuewen and Mei, Ting and Guo, Qi and Zhou, Wenjing and Li, Xiaomei and Chen, Jitao and Zhou, Xinke and Sun, Ning and Fang, Zhiyuan
Journal: Mikrochimica acta (2018): 2
Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
Authors: Stephen, Laurie and Schwarz, Emanuel and Guest, Paul C
Journal: Advances in experimental medicine and biology (2017): 149-156
Multiplex Immunoassay Profiling.
Authors: Stephen, Laurie
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 169-176
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404322C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404322C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 254 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY(3-36), PYY, human |
| 编码 | |
| 别名 | Peptide YY(3-36), PYY, human |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 |
| 序列（三字母缩写） | Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 |
| 基本描述 | Splenopentin, RKEVY, is an immunomodulatory pentapeptide corresponding to the amino acid residues 32-36 of the splenic hormone splenin. |
| 溶解度 | |
| 分子量 | 4049.6 |
| 化学式 | C180H279N53O54 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents |  |
| Figures |  |
| Reference | D. Grandt et al., Regulatory Peptides, 40, 161(1992) |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404321C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404321C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 203 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
 |
货号 | 1972 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 2 Labelings | 价格 | 2472 | |
| Ex (nm) | 651 | Em (nm) | 670 | |
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
ReadiLink™xtra快速抗体标记试剂是AAT 研发，由金畔代理销售的Cy5标记的抗体标记试剂盒。ReadiLink™xtra快速抗体标记试剂盒基本上只需要2个简单的混合步骤，而无需纯化。ReadiLink™试剂盒中使用的预活化Cy5非常稳定，对抗体表现出良好的反应性和选择性。该试剂盒具有用于标记〜2×50 ug抗体的所有基本成分。试剂盒中提供的两个预活化Cy5染料小瓶中的每一个均经过优化，可标记约50 µg抗体。ReadiLink™xtra Cy5快速抗体标记试剂盒提供了一种方便而强大的方法，可以使用红色的Cy5荧光团标记单克隆和多克隆抗体。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的抗体标记试剂盒。
点击查看光谱
产品说明书
实验方案
工作溶液配制
为了标记50 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将5 µL（反应总体积的10％）反应缓冲液（组分B）与50 µL目标蛋白质溶液混合。
注意：如果蛋白质浓度不同，请相应地调整蛋白质体积，以使〜50 µg蛋白质可用于标记反应。
注意：要标记100 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将10 µL（反应总体积的10％）反应缓冲液（组分B）与100 µL目标蛋白质溶液混合。
注意：蛋白质应溶于1X磷酸盐缓冲盐水（PBS），pH 7.2-7.4；如果蛋白质溶解在甘氨酸缓冲液中，则必须针对1X PBS（pH 7.2-7.4）进行透析，或使用10 kDa的Amicon Ultra-0.5，Ultracel-10膜去除游离的胺或铵盐（例如硫酸铵和乙酸铵）被广泛用于蛋白质沉淀。
注意：为获得最佳标记效率，建议最终蛋白质浓度范围为1-2 mg / mL，结合效率明显降低，低于1 mg / mL。
操作步骤
蛋白质缀合物应在载体蛋白（例如0.1％牛血清白蛋白）存在下以> 0.5 mg / mL的浓度存储。为了更长的存储时间，可以将蛋白质缀合物冻干或分成单份使用，并存储在≤–20°C下。
图示
图1. HeLa细胞中微管蛋白的免疫荧光染色。HeLa细胞用4％PFA固定，用0.1％Triton X-100透化并封闭。然后将细胞与小鼠抗微管蛋白抗体一起孵育，并用ReadiLink™xtra Rapid Cy5抗体标记试剂盒（#1972）标记的山羊抗小鼠IgG染色。
参考文献
Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
Authors: Sun, Weiping and Huo, Yinbo and Mei, Yuxuan and Zhou, Qingtong and Zhao, Suwen and Zhuang, Min
Journal: ACS chemical biology (2020): 39-43
Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
Authors: Noviana, Eka and Jain, Sidhartha and Hofstetter, Josephine and Geiss, Brian J and Dandy, David S and Henry, Charles S
Journal: Analytical and bioanalytical chemistry (2020): 3051-3061
A neuraminidase potency assay for quantitative assessment of neuraminidase in influenza vaccines.
Authors: Byrne-Nash, Rose T and Gillis, Jacob H and Miller, David F and Bueter, Katie M and Kuck, Laura R and Rowlen, Kathy L
Journal: NPJ vaccines (2019): 3
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.
Authors: Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald
Journal: Molecular imaging and biology (2019): 54-66
Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes.
Authors: Gao, Fenglei and Fan, Taotao and Ou, Shanshan and Wu, Jing and Zhang, Xing and Luo, Jianjun and Li, Na and Yao, Yao and Mou, Yingfeng and Liao, Xianjiu and Geng, Deqin
Journal: Biosensors & bioelectronics (2018): 201-208
Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Authors: Lu, Xuewen and Mei, Ting and Guo, Qi and Zhou, Wenjing and Li, Xiaomei and Chen, Jitao and Zhou, Xinke and Sun, Ning and Fang, Zhiyuan
Journal: Mikrochimica acta (2018): 2
Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
Authors: Stephen, Laurie and Schwarz, Emanuel and Guest, Paul C
Journal: Advances in experimental medicine and biology (2017): 149-156
Multiplex Immunoassay Profiling.
Authors: Stephen, Laurie
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 169-176
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY, PYY, canine, mouse, porcine, rat |
| 编码 | [81858-94-8] |
| 别名 | Peptide YY, PYY, canine, mouse, porcine, rat |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-NH2 |
| 序列（三字母缩写） | H-Tyr-Pro-Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2?(trifluoroacetate salt) |
| 基本描述 | Y2 receptor agonist, released from the body’s gastrointestinal tract in proportion to caloric intake, that significantly decreases appetite and reduces food intake, and thus may act as a drug for the treatment of obesity. |
| 溶解度 | |
| 分子量 | 4240.7 |
| 化学式 | C190H288N54O57 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents | ![Peptide YY, PYY, canine, mouse, porcine, rat 编码 [81858-94-8]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2da6db3e3.png) |
| Figures | ![Peptide YY, PYY, canine, mouse, porcine, rat 编码 [81858-94-8]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2da738f28.jpg) |
| Reference | K. Tatemoto et al., Nature, 285, 417 (1980) R. Corder et al., Regulatory Peptides, 21, 253 (1988) K. Tatemoto, PNAS, 79, 2514 (1982) |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 1988 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 2 Labelings | 价格 | 2472 |
| Ex (nm) | 752 | Em (nm) | 776 |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
ReadiLink™xtra快速抗体标记试剂是AAT 研发，由金畔代理销售的AF750标记的抗体标记试剂盒。ReadiLink™xtra快速抗体标记试剂盒基本上只需要2个简单的混合步骤，而无需纯化。ReadiLink™试剂盒中使用的预活化AF750非常稳定，对抗体表现出良好的反应性和选择性。该试剂盒具有用于标记〜2×50 ug抗体的所有基本成分。试剂盒中提供的两个预活化AF750染料小瓶中的每一个均经过优化，可标记约50 µg抗体。ReadiLink™xtra AF750快速抗体标记试剂盒提供了一种方便而强大的方法，可以使用近红外荧光的AF750荧光团标记单克隆和多克隆抗体。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的抗体标记试剂盒。
点击查看光谱
产品说明书
实验方案
工作溶液配制
为了标记50 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将5 µL（反应总体积的10％）反应缓冲液（组分B）与50 µL目标蛋白质溶液混合。
注意：如果蛋白质浓度不同，请相应地调整蛋白质体积，以使〜50 µg蛋白质可用于标记反应。
注意：要标记100 µg蛋白质（假设目标蛋白质浓度为1 mg / mL），请将10 µL（反应总体积的10％）反应缓冲液（组分B）与100 µL目标蛋白质溶液混合。
注意：蛋白质应溶于1X磷酸盐缓冲盐水（PBS），pH 7.2-7.4；如果蛋白质溶解在甘氨酸缓冲液中，则必须针对1X PBS（pH 7.2-7.4）进行透析，或使用10 kDa的Amicon Ultra-0.5，Ultracel-10膜去除游离的胺或铵盐（例如硫酸铵和乙酸铵）被广泛用于蛋白质沉淀。
注意：为获得最佳标记效率，建议最终蛋白质浓度范围为1-2 mg / mL，结合效率明显降低，低于1 mg / mL。
操作步骤
蛋白质缀合物应在载体蛋白（例如0.1％牛血清白蛋白）存在下以> 0.5 mg / mL的浓度存储。为了更长的存储时间，可以将蛋白质缀合物冻干或分成单份使用，并存储在≤–20°C下。
参考文献
Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
Authors: Sun, Weiping and Huo, Yinbo and Mei, Yuxuan and Zhou, Qingtong and Zhao, Suwen and Zhuang, Min
Journal: ACS chemical biology (2020): 39-43
Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
Authors: Noviana, Eka and Jain, Sidhartha and Hofstetter, Josephine and Geiss, Brian J and Dandy, David S and Henry, Charles S
Journal: Analytical and bioanalytical chemistry (2020): 3051-3061
A neuraminidase potency assay for quantitative assessment of neuraminidase in influenza vaccines.
Authors: Byrne-Nash, Rose T and Gillis, Jacob H and Miller, David F and Bueter, Katie M and Kuck, Laura R and Rowlen, Kathy L
Journal: NPJ vaccines (2019): 3
Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526
Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.
Authors: Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald
Journal: Molecular imaging and biology (2019): 54-66
Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes.
Authors: Gao, Fenglei and Fan, Taotao and Ou, Shanshan and Wu, Jing and Zhang, Xing and Luo, Jianjun and Li, Na and Yao, Yao and Mou, Yingfeng and Liao, Xianjiu and Geng, Deqin
Journal: Biosensors & bioelectronics (2018): 201-208
Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Authors: Lu, Xuewen and Mei, Ting and Guo, Qi and Zhou, Wenjing and Li, Xiaomei and Chen, Jitao and Zhou, Xinke and Sun, Ning and Fang, Zhiyuan
Journal: Mikrochimica acta (2018): 2
Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476
Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
Authors: Stephen, Laurie and Schwarz, Emanuel and Guest, Paul C
Journal: Advances in experimental medicine and biology (2017): 149-156
Multiplex Immunoassay Profiling.
Authors: Stephen, Laurie
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 169-176
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404264C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404264C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 457 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司可以定制不同序列多肽，可以访问官网了解更多产品信息。
| 名称 | Peptide YY, PYY, human |
| 编码 | [118997-30-1] |
| 别名 | Peptide YY, PYY, human |
| 纯度 | 80%，90%，95%，98%，99% |
| 重量 | 1mg,5mg,10mg,50mg,100mg,1g |
| 序列（单字母缩写） | YPIKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY-NH2 |
| 序列（三字母缩写） | H-Tyr-Pro-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2?(trifluoroacetate salt) |
| 基本描述 | |
| 溶解度 | |
| 分子量 | 4309.8 |
| 化学式 | C194H295N55O57 |
| 存储条件 | Store at -20°C. Keep tightly closed. Store in a cool dry place. |
| 注释 | |
| Documents | ![Peptide YY, PYY, human 编码 [118997-30-1]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2d9f1af01.png) |
| Figures | ![Peptide YY, PYY, human 编码 [118997-30-1]](http://www.saliva.com.cn/wp-content/uploads/2022/10/20221005_633d2d9f703f1.jpg) |
| Reference | K. Tatemoto et al., Nature, 285, 417 (1980) K. Tatemoto, PNAS, 79, 2514 (1982) |
| C端 | |
| N端 | |
| 化学桥 |
上海金畔生物科技有限公司提供具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404263C，可以访问官网了解更多产品信息。
具砂板闪式层析柱（带Rodaviss 磨口）,欣维尔,C404263C
产品简介：具砂板的Rodaviss 层析柱，每个层析柱标配一个四氟节门塞。
技术参数：
| 材质 | 玻璃 |
| 规格（mm） | 305 |
| 标准口 | 24/40 |
| 砂芯 | |
| 活塞 |
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | V101070 | 存储条件 | Multiple |
| 规格 | 96 Tests | 价格 | 11988 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Mabtech的经过仔细验证的ELISAPRO试剂盒提供了所有必需的试剂，以方便，可靠，特异的方式方便地定量测定血清，血浆和细胞培养上清液中的分析物。
ELISAPRO试剂盒随附有预先涂有单克隆抗体（mAb）。样品中的分析物被包被的mAb捕获，并被生物素化的检测mAb和链霉亲和素-HRP（SA-HRP）检测。注：TMB底物的添加将导致底物出现颜色。用硫酸终止反应，并且可以使用ELISA板酶标仪定量光密度。通过与平行分析的ELISA标准品的系列稀释液比较来确定分析物的浓度。
Stop溶液0.18 M H2SO4（<1％）对眼睛和皮肤有刺激性，应小心处理。由于不知道暴露的影响，因此应仔细处理该标准。溶液中的缓冲液和试剂含有防腐剂Kathon CG（0.002％），这是一种潜在的过敏原，可能通过皮肤接触引起过敏。人和动物样品应被视为潜在危险的生物材料。所有材料应按照当地法规进行处理。有关更多信息，请查阅我们网站上的《安全数据表》。
在开始测定之前，让板和测定试剂达到室温（TMB底物除外，最好使用冷底物）。
计划板布局，使其一式两份，包括标准曲线，样品和测定背景对照。每孔的体积不应超过100μl。
产品说明书
样品实验方案
储备溶液配制
将50 ml洗涤缓冲液浓缩液添加到950 ml蒸馏水或去离子水中（足以完成1个板的所有洗涤步骤）。如果在20倍浓缩物中形成了晶体，则将其升至室温并轻轻混合以溶解。
通过用蒸馏水或去离子水将样品稀释液浓缩液稀释5倍来准备所需体积的样品稀释液。对于每个板，将30 ml样品稀释液添加到120 ml水中。
所有样品均应在样品稀释液中稀释至少2倍。除去可见的沉淀物并在聚丙烯管/板中稀释，应在样品之前添加缓冲液。高度溶血和高血脂的样品可能会导致定量结果不准确。含有超出分析标准范围的高水平分析物的样品将需要进一步稀释。
根据对BALB / c和C57BL / 6样品的重复分析，我们建议稀释倍数为200,000X。精确的移液非常重要，在稀释步骤之间更换吸头，并使用新鲜制成的稀释液。试剂的含量足以重复使用。
通过添加1 ml标准重构缓冲液将ELISA标准液重构为1μg/ ml的原液。不要搅拌。让标准液溶解20分钟并彻底混合。标准液应等份保存在-20°C下。避免重复冻融循环。
如图所示，稀释标准储备溶液以创建标准曲线。指示的数量足以重复。最后一个样品瓶用作测定背景对照，即应省略标准液。在使用30分钟内准备标准曲线。
使用后15分钟内，将检测抗体在Apo ELISA缓冲液中稀释至1μg/ ml的浓度。对于每个板，在12 ml Apo ELISA缓冲液中稀释12μl检测抗体。
在使用后的15分钟内，将抗生蛋白链菌素-HRP用抗生蛋白链菌素-HRP稀释剂稀释1000倍。对于每块板，在12 ml的链霉亲和素-HRP稀释液中稀释12μl的链霉亲和素-HRP。