Buccutite FOL，马来酰亚胺 [MTAM]

	货号	5358	存储条件	在零下15度以下保存, 避免光照
	规格	2 umoles	价格	2388
	Ex (nm)		Em (nm)
	分子量	514.54	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：5358

产品名称：Buccutite FOL，马来酰亚胺 [MTAM]

规格：2 umoles

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

外观：固体

分子量：514.54

溶剂：DMSO

产品介绍

Buccutite 交联技术提供了方便、有效的交联方法，以高共轭产率连接两个生物分子。该方法使用一对交联剂：Buccutite MTA 和 Buccutite FOL。 MTA 被添加到一个分子中，而 FOL 被添加到另一个分子中。通过在中性条件下混合 Molecule-1-Buccutite MTA 和 Molecule-2-Buccutite FOL 来引发交联反应。Buccutite MTA 马来酰亚胺 (MTAM) 的使用方式与广泛用于交联蛋白质的 SMCC 相同。MTAM 的一端（通过马来酰亚胺）与还原抗体（通过 TCEP 或 DTT）中发现的半胱氨酸的硫醇 (-SH) 反应。 SMCC 交联需要高浓度的蛋白质。此外，SMCC 修饰的蛋白质极不稳定，通常会自我反应，因为蛋白质通常同时含有胺和硫醇基团，这会导致大量的同交联。 Buccutite 交联反应在极其温和和中性的条件下发生，无需任何催化剂，它比SMCC更加稳定。

参考文献

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