上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*
| 货号 | 17293 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 5 mL | 价格 | 3612 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | Water | |
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：17293
产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*
规格：5ml
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
溶剂：水
产品介绍
用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。
适用仪器
| qPCR | |
| 仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
| 成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 终浓度 |
| TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* | 12.5 µL | 25 µL | 1X |
| 上游引物，10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
| 下游引物，10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
| DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
| 无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
| 范围 | 聚合酶 | PCR (30-40个循环) | ||
| Hold | 变性 | 退火 | 延伸 | |
| 温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
| 时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9
[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6
[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70