ReadiUse CL-APC [交联 – 别藻蓝蛋白] *无硫酸铵* 货号2504-AAT Bioquest荧光染料

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ReadiUse CL-APC [交联 – 别藻蓝蛋白] *无硫酸铵*

ReadiUse CL-APC [交联 – 别藻蓝蛋白] *无硫酸铵*

ReadiUse CL-APC [交联 - 别藻蓝蛋白] *无硫酸铵*    货号2504 货号 2504 存储条件
规格 10 mg 价格 6564
Ex (nm) 651 Em (nm) 660
分子量 ~105000 溶剂
产品详细介绍

简要概述

产品基本信息

货号:2504

产品名称:ReadiUse CL-APC [交联 – 别藻蓝蛋白] *无硫酸铵*

规格:10mg

储存条件:2-8℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):651

发射波长(nm):662

 

产品介绍

ReadiUse CL-APC [交联 – 别藻蓝蛋白]是美国AAT Bioquest生产的蛋白标记染料,交联的别藻蓝蛋白(CL-APC)是从螺旋藻(一种蓝绿藻)中分离的藻胆蛋白。与其他藻胆蛋白一样,APC具有强荧光性,具有极高的吸收率和高量子效率。它是一种蛋白质,通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接,而不会改变其光谱特征。 APC在主要的藻胆蛋白中是最不稳定的,在低浓度下易于解离,包括进行某些测定的浓度。 CL-APC在α和β亚基之间发生化学交联,比APC稳定得多。交联的APC在水溶液中具有改善的稳定性。然而,所有商业APC材料都在浓硫酸铵缓冲液中出售。来自其他供应商的商业APC材料需要进行繁琐的透析或其他纯化,然后才能用于标记目的。 AAT Bioquest提供此ReadiUse APC,可轻松用于任何标签,无需任何纯化。我们高度纯化的ReadiUse APC促进APC与抗体和其他蛋白质(如链霉抗生物素蛋白和其他二级试剂)的快速结合。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse CL-APC [交联 – 别藻蓝蛋白]。 

点击查看光谱

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

说明书
ReadiUse CL-APC [交联 – 别藻蓝蛋白] *无硫酸铵*.pdf