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核紫 DCS1 死细胞染料
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货号 | 17549 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 0.5 ml | 价格 | 1164 | |
| Ex (nm) | 371 | Em (nm) | 454 | |
| 分子量 | 744.77 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：17549
产品名称：核紫 DCS1 死细胞染料
规格：0.5ml
储存条件：-20℃干燥
产品物理化学光谱特性
溶剂：DMSO
激发波长(nm)：371
发射波长(nm)：454
产品介绍
我们的Nuclear Violet DCS1是一种非荧光，DNA选择性和细胞不透性的紫色荧光染料，用于分析死细胞中的DNA含量。 核紫色DCS1在与双链DNA结合后具有明显增强的荧光。 它可用于荧光成像，微孔板和流式细胞仪应用。 这种DNA结合染料可用于死细胞的多色分析。
点击查看光谱
适用仪器
| 荧光显微镜 | |
| 激发： | DAPI |
| 发射: | DAPI |
| 推荐孔板： | 黑色透明孔板 |
产品说明书
染色细胞分析方案
注意：以下方案适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。
工作溶液配制
使用缓冲液将核紫 DCS1储备溶液（5mM）稀释到0.5-5μm的浓度。
操作步骤
1.将Nuclear Violet DCS1工作溶液加到固定、死亡或凋亡的细胞（悬浮液或粘附细胞）中，并将细胞染色15至60分钟。 在最初的实验中，最好尝试几种染料浓度来确定产生所需结果的最佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。
2.用荧光显微镜、荧光酶标仪或流式细胞仪直接检测染色细胞。
图示
图1.在96孔板上染色的固定和活（非固定）Hela细胞，与核蓝 DCS1 1μm孵育20分钟并在DAPI通道成像。
参考文献
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Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.
Authors: Wang, Qi and Rodrigues, Matthew A and Repin, Mikhail and Pampou, Sergey and Beaton-Green, Lindsay A and Perrier, Jay and Garty, Guy and Brenner, David J and Turner, Helen C and Wilkins, Ruth C
Journal: Radiation research (2019): 342-351
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