CIA 胶原诱导的关节炎模型
胶原诱导的关节炎模型 （CIA）
实验方案
1. 牛II型胶原溶解在0.05mol/ml的乙酸中，浓度为2mg/ml，搅拌4°C过夜。
2. 注射前，II胶原溶液与同体积的IFA在冰浴中*乳化。
3. *天，每只大鼠尾根部皮内注射0.1ml CII/IFA乳化剂（浓度为1mg/ml）。
4. 第7天，每只大鼠以同样的方式再次注射。
5. 注射后，大鼠关节炎指数根据肉眼评分系统每周两次进行评价。
0－正常
1－一个足爪肿胀和/或发红
2－2个相关关节肿胀和/或发红
3－>2个相关关节肿胀和/或发红
4－整个足爪严重炎症
每只大小鼠关节炎指数为每只动物4足爪得分之和。
6. 肿胀严重性根据器官充满度测定法通过气道容积的改变来测定， 一周两次。
1. 牛II型胶原溶解在0.05mol/ml的乙酸中，浓度为2mg/ml，搅拌4°C过夜。
2. 注射前，II胶原溶液与同体积的IFA在冰浴中*乳化。
3. *天，每只大鼠尾根部皮内注射0.1ml CII/IFA乳化剂（浓度为1mg/ml）。
4. 第7天，每只大鼠以同样的方式再次注射。
5. 注射后，大鼠关节炎指数根据肉眼评分系统每周两次进行评价。
0－正常
1－一个足爪肿胀和/或发红
2－2个相关关节肿胀和/或发红
3－>2个相关关节肿胀和/或发红
4－整个足爪严重炎症
每只大小鼠关节炎指数为每只动物4足爪得分之和。
6. 肿胀严重性根据器官充满度测定法通过气道容积的改变来测定， 一周两次。
Brief protocol of Collagen induced arthritis model （CIA）
1. Bovine CII is dissolved in 0.05mol/L acetic acid at a concentration of 2 mg/ml by stirring overnight at 4°C.
2. Before injection, CII is emulsified with an equal volume of IFA in an ice-cold water bath.
3. On day1, each rat is injected intradermally at the base of the tail with 0.1ml CII/IFA the emulsion （1mg/ml）.
4. On day 21, a second injection of CII in IFA will be administered using the same amount.
5. After the second injection，rats will be evaluated daily for arthritis using a macroscopic scoring system of 0–4.
0 no signs of arthritis
1 swelling and/or redness of the paw or 1 digit
2=2 joint involved
3=>2 joint involved
4severe arthritis of the entire paw and digits
An arthritis index for each rat is calculated by adding the scores for each of the 4 paws.
6. Swelling severity is also determined by quantitating the change in the paw volume, as measured by plethysmometry.
1. Bovine CII is dissolved in 0.05mol/L acetic acid at a concentration of 2 mg/ml by stirring overnight at 4°C.
2. Before injection, CII is emulsified with an equal volume of IFA in an ice-cold water bath.
3. On day1, each rat is injected intradermally at the base of the tail with 0.1ml CII/IFA the emulsion （1mg/ml）.
4. On day 21, a second injection of CII in IFA will be administered using the same amount.
5. After the second injection，rats will be evaluated daily for arthritis using a macroscopic scoring system of 0–4.
0 no signs of arthritis
1 swelling and/or redness of the paw or 1 digit
2=2 joint involved
3=>2 joint involved
4severe arthritis of the entire paw and digits
An arthritis index for each rat is calculated by adding the scores for each of the 4 paws.
6. Swelling severity is also determined by quantitating the change in the paw volume, as measured by plethysmometry.
订货信息：021-50837765
| 20011 | Immunization Grade Chick Type II Collagen | 2775 | chondrex |
| 20012 | Immunization Grade Chick Type II Collagen, Solution | 2775 | chondrex |
| 20021 | Immunization Grade Bovine Type II Collagen | 2775 | chondrex |
| 20022 | Immunization Grade Bovine Type II Collagen, Solution | 2775 | chondrex |
| 20031 | Immunization Grade Porcine Type II Collagen | 2775 | chondrex |
| 20032 | Immunization Grade Porcine Type II Collagen, Solution | 2775 | chondrex |
| 20041 | Immunization Grade Rat Type II Collagen | 4335 | chondrex |
| 20042 | Immunization Grade Rat Type II Collagen, Solution | 4335 | chondrex |
| 20051 | Immunization Grade Human Type II Collagen | 4590 | chondrex |
| 20052 | Immunization Grade Human Type II Collagen, Solution | 4590 | chondrex |
| 20061 | Immunization Grade Mouse Type II Collagen | 2340 | chondrex |
| 20062 | Immunization Grade Mouse Type II Collagen, Solution | 2340 | chondrex |
| 20071 | Immunization Grade Goat Type II Collagen | 3075 | chondrex |
| 20081 | Immunization Grade Sheep Type II Collagen | 3075 | chondrex |
| 2011 | ELISA Grade Chick Type II Collagen | 2400 | chondrex |
| 2012 | ELISA Grade Bovine Type II Collagen | 2400 | chondrex |
| 2013 | ELISA Grade Porcine Type II Collagen | 2400 | chondrex |
| 2014 | ELISA Grade Rat Type II Collagen | 2910 | chondrex |
| 2015 | ELISA Grade Human Type II Collagen | 4785 | chondrex |
| 2016 | ELISA Grade Mouse Type II Collagen | 6750 | chondrex |
| 2017 | ELISA Grade Monkey Type II Collagen | 3000 | chondrex |
| 2021 | T-Cell Proliferation Grade Chick Type II Collagen | 2400 | chondrex |