viagenbiotech 501-PK 说明书

世界*实验材料供应商 Viagen Biotech, Inc.上海金畔生物为其中国代理， Viagen Biotech, Inc.在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Viagen Biotech, Inc.就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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Viagen Biotech Inc.是创新的DNA提取技术和生命科学研究产品的供应商。ViagenDirectPCR®DNA提取系统是一种单管系统，用于从小鼠尾巴，耳朵片，卵黄囊和培养细胞中快速制备DNA。由Viagen Biotech Inc.的科学家开发的正在申请的组件允许得到的DNA提取物与用于基因分型的基因组PCR相容。生物样品的粗提取物与许多分子生物学级反应（如聚合酶链反应（PCR））不相容，部分原因是粗提物中含有抑制剂。DirectPCR试剂不仅介导生物样品的快速溶解，而且还含有有效抑制粗裂解物对PCR扩增的抑制活性的抑制剂，同时zui大限度地保持了释放的基因组DNA的完整性。该过程*消除了任何溶液转移或开管步骤，节省了大量的时间和精力。DirectPCR试剂每尾只花费26美分，可以节省任何使用量。

Viagenbiotech致力于简便快速提取和制备核酸（DNA / RNA），以批量、快速进行基因分析。公司拥有的技术不仅加快样品的裂解，而且降低了粗提物中PCR抑制因素，因而使DNA提取与PCR基因分析兼容，样品经一步消化后即可进行PCR扩增，并且保证了基因组的完整性。

Proteinase K Solution 5ml (20mg/ml)

Availability: In stock

Quick Overview

Genomic PCR Grade Proteinase K Solution 

Catalog No. 501-PK

Stable for 12 months at -20℃ / Ships Refrigerated Fedex Overnight

Details

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.

DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.

Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.

Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C

Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.

Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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