• High transfection efficiency and significantly lower toxicity levels for a wide range of cell lines
• Significantly improved transfection performance in a number of primary and hard-to-transfect cell lines
• Optimized protocols for over 30 different cell lines are available so much less time is needed for evaluation and optimization

• Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells
• Used for a large-scale transient protein production with milligrams of protein yield

• Superior transfection efficiency requiring lower RNAi concentrations leading to more effective gene knockdown with minimal non-specific effects
• Easy optimization due to minimal cytotoxicity across a 10-fold concentration range of transfection reagent
• Compatibility with a broad range of cell types providing the most versatile approach to all of your gene silencing experiments

• Achieves highest efficiency and expression results for plasmid or RNAi transfections
• Works effectively with many cell types (adherent or suspension)
• Easy and fast protocol – no need for wash steps before or after transfection
• Complexes can be added to cells growing in serum-containing media
• Ideal if transfecting at confluencies of 90% or greater (minimizes cytotoxicity following transfection)

•  Same performance as Lipofectamine™ 2000, certified animal-origin free (“CD” = chemically defined)

• Transfection of antisense oligonucleotides

• Used for transient protein production in combination with the FreeStyle™ 293 Expression System
• Optimized for suspension FreeStyle™ 293-F cells

• Optimal transfection of insect cells, including S2, Sf9, Sf21 and High Five™ cells

• Transfection of suspension cells, including CHO, lymphoid and Jurkat cell lines.

• First generation reagent for plasmid DNA transfections
• In most cases, Lipofectamine™ 2000 provides better performance than Lipofectamine™ Transfection Reagent

• First generation reagent for plasmid DNA transfections
• In most cases, Lipofectamine™ 2000 provides better performance than Lipofectin® Transfection Reagent

• Broad use reagent designed for low confluency applications (<70% confluent at the time of transfection)
• Useful for cell lines that are sensitive to transfection reagents

To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents to mediate efficient delivery into the cell's nucleus. This process is essential for subsequent protein expression of the gene of interest. Plasmids are small circular DNA molecules that naturally occur in bacteria, and are actually used by the bacteria to transfer genetic information. The mechanism of adding a DNA Plasmid into a mammalian cell is known as plasmid transfection.

Invitrogen offers a wide range of transfection reagents for your specific needs.  For cell types specific protocols, please refer to Transfection Selection Tool.

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  • Plasmid Transfection Reagents
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