SeqPure PCR纯化试剂盒

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SeqPure PCR纯化试剂盒

BioChain成立于1994年，总部位于旧金山湾区。

BioChain是高质量加工生物样品产品和分析服务的先进者，用于推进转化医学，伴随诊断和临床产品研发。BioChain的产品可用于DNA和RNA测序，PCR和RT-PCR，基因表达分析，DNA和RNA纯化，蛋白质提取和纯化以及蛋白质表达分析。BioChain的下一代测序一站式服务，从临床样品采集，样品制备，测序到生物信息学分析，将*改变行业。

除了我们*的生物标本和样品制备业务，BioChain正在进入临床应用领域，为癌症和生殖健康提供临床基因组学诊断产品和临床试验服务。BioChain致力于通过持续流程改进计划以竞争力的价格实现高质量的产品和服务。BioChain建立了全面的质量保证计划，以满足客户的满意度，并为我们的内部生产设施保持ISO 9001：2015和ISO 13485：2003认证。

 

SeqPure PCR纯化试剂盒（5 ml）

SeqPure PCR Purification Kit (5 ml)

 

概观

去除引物，酶，短DNA段，盐和其他缓冲液成分可提高从PCR片段获得的DNA序列数据的质量。BioChain的SeqPure提供了一种，方便且经济的方法来清除长度超过100bp的DNA。

描述

SeqPure™PCR试剂是一种基于顺磁珠技术的后PCR和下一代文库制备清洗系统，旨在有效纯化PCR扩增子。纯化包括除去盐，引物，引物二聚体，dNTP，因为DNA段选择性地与磁珠颗粒结合; 用低盐洗脱缓冲液或水洗脱高纯度的DNA，可直接用于下游应用。该协议可以使用您当前的协议适应您当前的液体处理工作站（例如Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf），也可以手动执行。  

应用 

•PCR•突变检测和基因分型•测序（Sanger和下一代）•微阵列•限制酶清除•克隆•片段分析  

SeqPure PCR纯化试剂盒的特点/优点：

•与主要竞争对手的性能相似，价格低廉•在半小时内净化PCR产品•去除盐，dNTP，引物和酶•无需离心或真空过滤•无花式缓冲液; 只需使用乙醇和Tris-HCI / TE•易于自动化•适用于96孔和384孔格式•可扩展：只使用尽可能多的珠子  

 

 

DNA段的恢复性

用SeqPure珠和Ampure珠纯化等量的含有盐和蛋白质的100bp梯子。使用不同的缓冲液TE和预热的TE（用于增加产率）进行洗脱。加载洗脱的DNA（各8μl）并在1％琼脂糖凝胶上电泳。凝胶显示出3000-100bp的所有片段的优异回收率。                

 

有效的尺寸选择

 

 

简单协议：

 

 

的DNA纯化

SeqPure和AmPure在纯化含有盐和蛋白质的100 bp DNA梯中的比较。通过分光光度法测量纯度。260/280和260/230吸光度比均表明SeqPure可有效去除盐和蛋白质，与AmPure相当。  

 

关于SeqPure PCR纯化试剂盒的更多信息

对于许多分子生物学技术，从DNA中去除污染物对下游应用至关重要。例如，为了确保成功的测序反应，必须从模板中除去所有过量的污染物，例如引物，短缺失的PCR产物，未掺入的dNTP，盐和残留的蛋白质。这些试剂可以干扰后续反应并导致不可读的序列。虽然去除多余的污染物是产生高质量数据的关键，但这样做可能非常耗时。PCR纯化试剂盒可以方便地清除DNA，可以显着提高实验室的生产率。可以手动使用的小样本套件，同时可以在各种平台上轻松实现自动化，确保所有样品通量都采用相同的程序 – 确保一致的性能。这样的试剂盒也应该是有效的，并且以小的损失纯化DNA段。后，它应该具有成本效益。 BioChain的SeqPure PCR纯化试剂盒专为快速有效纯化PCR产物而设计。该系统利用顺磁珠技术选择性地结合100bp和更大尺寸的DNA段。在洗脱纯化的PCR产物之前，简单的洗涤步骤将除去盐和其他缓冲液成分，引物，引物二聚体，核苷酸和酶。SeqPure避免使用旋转柱和其他纯化膜，以便使试剂盒便于手动使用或自动化广泛的实验室自动化选择（例如KingFisher，Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf）中。96-和384-井格式可以很容易地适应这种技术。用顺磁颗粒而不是旋转柱进行纯化可以减少水动力剪切 – 提高较大DNA段的完整性。顺磁珠技术的可扩展性也使该套件更加灵活。如果您正在净化少量DNA，您可以使用更少的珠子，并为每个纯化试剂盒提取更多样品。

  • 目录号： Z1510001

 

  • 大小： 5毫升

 

 

 

K5011610

cfPure® Cell Free DNA Extraction Kit (100 ml)

1 Kit (100 ml)

biochain

K5011625

cfPure® Cell Free DNA Extraction Kit (250 ml)

1 Kit (250 ml)

biochain

K5011625MA

cfPure® MAX Cell-Free DNA Extraction Kit

1 Kit (250 ml)

biochain

Z1510001

SeqPure PCR Purification Kit (5 ml)

5 ml

biochain

Z1510002

SeqPure PCR Purification Kit (50 ml)

50 ml

biochain

Z1510003

SeqPure PCR Purification Kit (500 ml)

500 ml

biochain

L5011001

PureSil-Silica Magnetic Beads

1 L

biochain

L5011010

PureSil-Silica Magnetic Beads

10 mL

biochain

Z1322006

AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria

1 kit (48 extractions)

biochain

Z1322002

AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml

1 kit (48 extractions)

biochain

Z1322001

AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl

1 kit (48 extractions)

biochain

Z1322005

AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells

1 kit (48 extractions)

biochain

Z1322009

AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples

1 kit (48 extractions)

biochain

Z1322010

AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples

1 kit (48 extractions)

biochain

Z1322007

AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples

1 kit (48 extractions)

biochain

Z1322008

AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum

1 kit (48 extractions)

biochain

Z1322004

AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains

1 kit (48 extractions)

biochain

Z1322015

AnaPrep Total RNA Extraction Kit

1 kit

biochain

Z1322017

AnaPrep Total RNA Extraction Kit with DNase-Treatment

1 kit

biochain

Z1322003

AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma

1 kit (48 extractions)

biochain

Z1322011

AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A

1 kit

biochain

Z1322012

AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B

1 kit

biochain

T2235090-SB

CancerSeq™ Paraffin Tissue Tumor Block: Colon

1 Block

biochain

T2235152-SB

CancerSeq™ Paraffin Tissue Tumor Block: Lung

1 Block

biochain

T2235086-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Breast

5 Slides

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235152-SC

CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung

5 Curls

biochain

T2235086-SB

CancerSeq™ Paraffin Tissue Tumor Block: Breast

1 Block

biochain

T2235218A-SB

CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma

1 Block

biochain

T2235090-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Colon

5 Slides

biochain

T2235152-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Lung

5 Slides

biochain

T2235218A-ST

CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma

5 Slides

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235086-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

T2235248-SC

CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach

5 Curls

biochain

Z1510001

SeqPure PCR Purification Kit (5 ml)

5 ml

biochain

Z1510002

SeqPure PCR Purification Kit (50 ml)

50 ml

biochain

Z1510003

SeqPure PCR Purification Kit (500 ml)

500 ml

biochain

L1271010

T4 RNA Ligase 1

1000 Units

biochain

L1271050

T4 RNA Ligase 1

5000 Units

biochain

L1271500

T4 RNA Ligase 1

50000 Units

biochain

L1270010

T4 RNA Ligase 2, truncated

1000 Units

biochain

L1270500

T4 RNA Ligase 2, truncated

50000 Units

biochain

KS072012-I

Aligner 1-12

12

biochain

KS072012-II

Aligner 13-24

1 Kit

biochain

KS072012-III

Aligner 25-36

1 Kit

biochain

KS072012-IV

Aligner 37-48

1 Kit

biochain

KS071012-12

Gel Breaker Tubes

12

biochain

KS071012-11

Gel Cutter

12

biochain

KS071012-13

Gel Filter

12

biochain

KS073012-I

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12

1 Kit

biochain

KS073012-II

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24

1 Kit

biochain

KS073012-III

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36

1 Kit

biochain

KS073012-IV

RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48

1 Kit

biochain

KS073012

RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner

1 Kit

biochain

KS073012-T

RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners

1 Kit

biochain

KS074012-I

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12

1 Kit

biochain

KS074012-II

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24

1 Kit

biochain

KS074012-III

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36

1 Kit

biochain

KS074012-IV

RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48

1 Kit

biochain

KS074012

RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners

1 Kit

biochain

KS074012-T

RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners

1 Kit

biochain

K5017100

Blood and Serum DNA Isolation Kit

1 kit

biochain

K5011610

cfPure® Cell Free DNA Extraction Kit (100 ml)

1 Kit (100 ml)

biochain

K5011625

cfPure® Cell Free DNA Extraction Kit (250 ml)

1 Kit (250 ml)

biochain

K5011625MA

cfPure® MAX Cell-Free DNA Extraction Kit

1 Kit (250 ml)

biochain

K5019100

FFPE Tissue DNA Extraction Kit

1 kit

biochain

K5011350

FFPE Tissue DNA Extraction Kit – Column

1 kit

biochain

K5011450

FFPE Tissue DNA Extraction Kit — Magnetic Beads

1 kit

biochain

K5016005

Genomic DNA Extraction Kit

1 kit

biochain

K5011050

Saliva DNA Isolation Kit

1 kit

biochain

K5018100

Serum DNA Isolation Kit

1 kit

biochain

K5011250

Stool DNA Isolation Kit

1 kit

biochain

K5011150

Urine DNA Isolation Kit

1 kit

biochain

Z5060009

xMag-Streptavidin Microparticles

1 ml

biochain

T6234701-1

FDA Standard Frozen Tissue Array – Human Adult Normal

2 slides

biochain

T6234701-2

FDA Standard Frozen Tissue Array – Human Adult Normal

4 slides

biochain

T6334701-1

FDA Standard Frozen Tissue Array – Mouse Normal

2 slides

biochain

T6334701-2

FDA Standard Frozen Tissue Array – Mouse Normal

4 slides

biochain

T6434701-1

FDA Standard Frozen Tissue Array – Rat Normal

2 slides

biochain

T6434701-2

FDA Standard Frozen Tissue Array – Rat Normal

4 slides

biochain

T6235201

Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls

1 slide

biochain

T6235086-1

Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235086-5

Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235090-1

Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235090-5

Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235142-1

Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235142-5

Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235183-1

Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls

1 slide

biochain

T6235183-5

Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls

5 slides

biochain

T6235152-1

Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls

1 slide

biochain

T6235152-5

Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls

5 slides

biochain

T6734700-G02-1

Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6734700-G02-5

Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6734700-G01-1

Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6734700-G01-5

Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6234700-1

Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6234700-5

Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6235700-1

Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I

1 slide

biochain

T6235700-5

Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I

5 slides

biochain

T6834700-G02-1

Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6834700-G02-5

Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6834700-G01-1

Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I

1 slide

biochain

T6834700-G01-5

Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I

5 slides

biochain

T6134035-1

Multiple Species Frozen Tissue Array – Brain

1 slide

biochain

T6134035-5

Multiple Species Frozen Tissue Array – Brain

5 slides

biochain

T6134149-1

Multiple Species Frozen Tissue Array – Liver

1 slide

biochain

T6134149-5

Multiple Species Frozen Tissue Array – Liver

5 slides

biochain

 

 

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